DNA damage and glutathione content in radiology technicians

Background: We wanted to investigate the effect of X-rays on mononuclear blood cells (MNCs) and red blood cells (RBCs) of radiology technicians exposed to X-rays in hospital. Methods: DNA damage was detected by fluorometric analysis of DNA unwinding. Glutathione levels were measured with enzymatic m...

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Bibliographic Details
Published in:Clinica chimica acta 2003-10, Vol.336 (1), p.13-18
Main Authors: Akköse, Ayfer, Ömer, Beyhan, Yiğitbaşı, Abdullah
Format: Article
Language:English
Subjects:
Adolescent
Adult
Allied Health Personnel
Biological and medical sciences
Case-Control Studies
Catalase
Catalase - blood
DNA - radiation effects
DNA Damage
Erythrocytes - metabolism
Glutathione
Glutathione - blood
Humans
Lymphocytes - metabolism
Lymphocytes - radiation effects
Malondialdehyde
Malondialdehyde - blood
Medical sciences
Miscellaneous
Occupational Diseases - blood
Occupational Diseases - etiology
Occupational Diseases - genetics
smoking
Smoking - adverse effects
Smoking - blood
Technology, Radiologic
Traumas. Diseases due to physical agents
X-ray
X-Rays
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Summary:Background: We wanted to investigate the effect of X-rays on mononuclear blood cells (MNCs) and red blood cells (RBCs) of radiology technicians exposed to X-rays in hospital. Methods: DNA damage was detected by fluorometric analysis of DNA unwinding. Glutathione levels were measured with enzymatic method in mononuclear blood cells. Glutathione content and catalase (CAT) activity of erythrocytes, and plasma malondialdehyde (MDA) levels were determined by spectrophometric methods. Results: An insignificant increase in plasma malondialdehyde levels and a significant decrease in mononuclear blood cells glutathione levels were observed in nonsmoking radiology technicians. In smoking radiology technicians, on the other hand, in addition to an increase in plasma malondialdehyde levels, DNA damage was also significantly apparent. Besides mononuclear blood cells' glutathione depletion, the glutathione content of red blood cells was also found to be decreased. Conclusion: It can be suggested that smoking seems to augment the toxic effects of radiation.
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(02)00421-7