Adrenergic agonists induce heterologous sensitization of adenylate cyclase in NS20Y-D 2L cells

Adenylate cyclase activity in NS20Y cells expressing D 2L dopamine receptors was examined following chronic treatment with norepinephrine and epinephrine. Initial acute experiments revealed that both norepinephrine and epinephrine inhibited forskolin-stimulated cyclic AMP accumulation via D 2 recept...

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Bibliographic Details
Published in:FEBS letters 2001-05, Vol.497 (2), p.85-89
Main Authors: Johnston, Christopher A, Cumbay, Medhane G, Vortherms, Timothy A, Watts, Val J
Format: Article
Language:English
Subjects:
D 2 dopamine receptor
Epinephrine
Heterologous sensitization
Norepinephrine
NS20Y neuroblastoma
Supersensitization
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Summary:Adenylate cyclase activity in NS20Y cells expressing D 2L dopamine receptors was examined following chronic treatment with norepinephrine and epinephrine. Initial acute experiments revealed that both norepinephrine and epinephrine inhibited forskolin-stimulated cyclic AMP accumulation via D 2 receptors. Furthermore, chronic (18 h) activation of D 2 dopamine receptors by norepinephrine or epinephrine induced a marked increase (>10-fold) in subsequent forskolin-stimulated cyclic AMP accumulation. This heterologous sensitization of adenylate cyclase activity was blocked by D 2 dopamine receptor antagonists and by pertussis toxin pretreatment. In contrast, concurrent activation of Gα s or adenylate cyclase did not appear to alter noradrenergic agonist-induced sensitization.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)02442-5