Hydroxyl radical Production in the Penumbra Cortex following the middle cerebral artery thrombosis in Rat

• Published in: Japanese Journal of Pharmacology 1996, Vol.71 (suppl.2), p.322-322
• Main Authors: Mizuno, Atsuhiro, Umemura, Kazuo, Shimakura, Akira, Shimazu, Yoshihiro, Nakashima, Mitsuyoshi
• Format: Article
• Language: eng ; jpn
• ISSN: 0021-5198
• DOI: 10.1016/S0021-5198(19)37526-2

Oxygen free radical formation and subsequent lipid peroxidation have been postulated to be important steps in the pathogenesis of ischemic or hypoxic brain injury. We have investigated that hydroxyl radical production in the penumbra lesions after thrombotic occlusion of the middle cerebral artery (MCA) in male Wistar rats. 2.3-DHBA (dihydroxybenzoic acid) as an index of hydroxyl radical production was monitored by high-performance liquid chromatography with electrochemical detection using microdialysis technique up to 120 min following MCA occlusion in anesthetized with halothane. Microdialysis system was perfused with Ringers Solution containing 5mM salicylate at a flow rate of 2μL. The concentration of 2.3-DHBA gradually increased following MCA occlusion and continued at high level during observation period. MCI-186, a hydroxyl radical scavenger, which administrated as an intravenous infusion, significantly inhibited 2.3-DHBA production. Protective effect of MCI-186 was estimated for measuring with TTC staining 24 hr after MCA occlusion. MCI-186 significantly decreased the infarction size in cerebral cortex. These results suggest that production of reactive oxygen species may involve in the development of cerebral ischemic injury in penumbra lesion.