In vitro radical scavenging activities of newly synthesized polyphenols

• Published in: Japanese Journal of Pharmacology 2000, Vol.82 (suppl.2), p.240-240
• Main Authors: Imai, Keiko, Inagaki, Masahiro, Yamazaki, Yukako, Yura, Akihiko, Sasuga, Keiko, Morio, Yuri, Suzuki, Masako, Oguchi, Katsuji
• Format: Article
• Language: eng ; jpn
• ISSN: 0021-5198
• DOI: 10.1016/S0021-5198(19)48424-2

Novel phenetyl ether derivatives (PhE-d) and cinnamic amide derivatives (CA-d) have been synthesized(Tumura & Co.). As these compounds contain two mono- or polyphenol rings whose carbon chains are bound either with an ether (PhE-d) or amide bond (CA-d), we evaluated their radical scavenging activities in vitro at concentrations of 10^-6 ～ 10^-3 M using ESR spectroscopy with a spin-trap 5, 5-dimethyl-1-pyrroline N-oxide. The superoxide radical was generated with hypoxanthine-xanthine oxidase system. (Results)Scavenging activities were found to be closely associated with the positions of hydroxyl groups in benzene rings. The activity was largest with the compounds having two catechol rings followed by those with a catechol ring combined with a monophenol ring, and those with only monophenol rings did not show scavenging activities. Those with an amide bond showed larger scavenging activities than those with an ether bond. (Discussion)The findings are consistent with already known scavenging activities of catecholamines. These results suggest that PhE-d and CA-d have the potential to be a medicine for conditions associated with free radicals.