Modification of low-density lipoprotein by diesel exhaust particles

• Published in: Japanese Journal of Pharmacology 1994, Vol.64 (suppl.1), p.96-96
• Main Authors: Shitashige, Miki, Ikeda, Masahiko, Suzuki, Motohisa, Watarai, Ken, Sagai, Masaru, Tomita, Takako
• Format: Article
• Language: eng ; jpn
• ISSN: 0021-5198
• DOI: 10.1016/S0021-5198(19)50026-9

It is considered that diesel exhaust particles (DEP) may be responsible for pathogenesis of pulmonary diseases such as asthma and chronic bronchitis, but the mechanism remains obscure. Since alveoli are close to capillaries, soluble materials and oxygen radicals from DEP may be able to afftect endothelial cells, blood cells and blood components. In this study, modification of low-density lipoprotein by DEP was examined. DEP were collected from exhausts of four cylinder diesel engines (2,740cc) by glass filter. LDL were prepared from porcine serum by discontinuous ultracentrifugation, and incubated with DEP for 1 hr at 37℃. DEP (1, 10, 100 μg/ml)-treated LDL increased conjugated diene formation and negative charge, DEP dose-dependently. Uptake of DEP-treated LDL in macrophage (J774) was significantly increased in comparison with that of normal LDL by 18hr incubation. Cellular cholesterol ester accumulation in J774 was also increased by incubation with DEP-treated LDL. These results suggest that LDL are modified by incubation with DEP, and DEP-modified LDL may be able to cause the pulmonary inflammation.