Oxidizing intermediates in the reaction of ferrous EDTA with hydrogen peroxide. Reactions with organic molecules and ferrocytochrome c

The reaction between hydrogen peroxide and ferrous EDTA generates an oxidizing intermediate (I1) which is not the hydroxyl radical. It oxidizes ferrocytochrome c and also reacts with hydrogen peroxide (k5 = 3.2 X 10(3) M-1 S-1) to form a second oxidizing transient (I2). I1 is not scavenged by t-buty...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1986-05, Vol.261 (15), p.6730-6733
Main Authors: Rush, J D, Koppenol, W H
Format: Article
Language:English
Subjects:
Amino Acids
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Cytochrome c Group - metabolism
Edetic Acid
Ferrous Compounds
Free Radicals
Fundamental and applied biological sciences. Psychology
Hemoproteins
Hydrogen Peroxide
Hydroxides
Hydroxyl Radical
Iron
Iron Chelating Agents
Kinetics
Metalloproteins
Oxidation-Reduction
Proteins
Spectrophotometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The reaction between hydrogen peroxide and ferrous EDTA generates an oxidizing intermediate (I1) which is not the hydroxyl radical. It oxidizes ferrocytochrome c and also reacts with hydrogen peroxide (k5 = 3.2 X 10(3) M-1 S-1) to form a second oxidizing transient (I2). I1 is not scavenged by t-butyl alcohol whereas I2 is. I1 is found to be significantly less reactive than the hydroxyl radical toward benzoate ion, t-butyl alcohol, acetate ion, arginine, and serine, but is scavenged by compounds with readily oxidizable functional groups such as ethanol and isopropyl alcohol. This indicates that I1 does not undergo the characteristic reactions of the hydroxyl radical but shows a pattern of reactivity more associated with a metal ion oxidant like a ferryl (FeO2+)-EDTA complex.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)62677-3