A study of the separation of enantiomers of some aromatic carboxylic acids by high-performance liquid chromatography on a β-cyclodextrin-bonded stationary phase

The enantiomers of some aromatic carboxylic acids of biomedical interest were separated by high-performance liquid chromatography on a β-cyclodextin-bonded stationary phase. Evaluation of the system shows that the structure of the acids plays an important role in the possibility for separation of th...

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Bibliographic Details
Published in:Journal of Chromatography A 1985, Vol.333 (1), p.59-68
Main Authors: Feitsma, Karla G., Bosman, Jan, Drenth, Ben F.H., de Zeeuw, Rokus A.
Format: Article
Language:English
Subjects:
Analysis
Applied sciences
Biological and medical sciences
Exact sciences and technology
General pharmacology
Medical sciences
Other techniques and industries
Pharmacology. Drug treatments
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Summary:The enantiomers of some aromatic carboxylic acids of biomedical interest were separated by high-performance liquid chromatography on a β-cyclodextin-bonded stationary phase. Evaluation of the system shows that the structure of the acids plays an important role in the possibility for separation of the enantiomers. Moreover, the pH as well as the buffer components of the mobile phase have a pronounced effect on the retention times and the selectivity of the system. In order to reduce tailing, the column temperature was increased; this resulted in better peak resolution. Anomalous behaviour was seen in the relation between retention time and sample concentration. At low amounts of injected sample the retention times increased, but this could not be ascribed to adsorption processes. This phenomenon caused great problems in the quantitative analysis of the two enantiomers with this system.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)87324-6