Determination of selenium in organic compounds on a silica gel sintered thin-layer chromatographic plate with 2,3-diaminonaphthalene after direct digestion

Biologically interesting selenium compounds such as selenomethionine, selenocystine and trimethylselenonium were digested directly on a silica gel sintered thin-layer chromatographic (TLC) plate. Digestion was performed with nitric acid—perchloric acid (2:1, v/v) by heating at 210°C for 15 min. Sele...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 1991, Vol.537 (1-2), p.397-405
Main Authors: Hasunuma, Ryoichi, Ogawa, Tadao, Ishii, Jukichi, Kawanishi, Yasuhiro
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Inorganic compounds
Other biological molecules
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Biologically interesting selenium compounds such as selenomethionine, selenocystine and trimethylselenonium were digested directly on a silica gel sintered thin-layer chromatographic (TLC) plate. Digestion was performed with nitric acid—perchloric acid (2:1, v/v) by heating at 210°C for 15 min. Selenite formed on the plate was revealed with 2,3-diaminonaphthalene reagent and determined using a TLC scanner. The recoveries of selenomethionine, selenocystine and trimethylselenonium were 94±7, 99±9 and 97±10%, respectively, with a detection limit of 0.4 ng of selenium. Linearity between fluorescence intensity and selenium content was found in the range 3–250 ng of selenium.
ISSN:0021-9673
DOI:10.1016/S0021-9673(01)88913-5