Comparative adsorption of adenine and nicotinamide adenine dinucleotide (NAD +) at an aqueous solution/mercury interface

The relative extent of adsorption of adenine and NAD + at the 10-μ M level from a solution of pH. 4.8 and 0.5 M ionic strength (McIIvaine buffer) has been measured as a function of potential by an inverse normal pulse polarographic technic at a dropping mercury electrode (d.m.e.). Normalization of t...

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Bibliographic Details
Published in:Journal of electroanalytical chemistry and interfacial electrochemistry 1983, Vol.156, p.299-306
Main Authors: Jensen, Mark A., Bresnahan, William T., Elving, Philip J.
Format: Article
Language:English
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Summary:The relative extent of adsorption of adenine and NAD + at the 10-μ M level from a solution of pH. 4.8 and 0.5 M ionic strength (McIIvaine buffer) has been measured as a function of potential by an inverse normal pulse polarographic technic at a dropping mercury electrode (d.m.e.). Normalization of the pulse polarographic current resulting from pre-concentration of the electroactive species through adsorption compared to the current expected for a simple diffusion-controlled electron-transfer indicates that NAD + is strongly adsorbed over a broader potential range than adenine, and that its surface concentration is slightly greater; causes for such behavior are considered. Other normalization approaches indicate NAD + to be strongly adsorbed but comparably to adenine when account is taken of the relative effective areas occupied on adsorption.
ISSN:0022-0728
DOI:10.1016/S0022-0728(83)80681-0