Infrared spectroscopic study of cervical smears in patients with HIV: Implications for cervical carcinogenesis

• Published in: The Journal of laboratory and clinical medicine 2000, Vol.135 (1), p.26-31
• Main Authors: Rigas, Basil, Laguardia, Katherine, Qiao, Liang, Bhandare, Prashant S., Caputo, Thomas, Cohenford, Menashi A.
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - complications; Adenocarcinoma - diagnosis; Adenocarcinoma - metabolism; Adolescent; Adult; Biological and medical sciences; Female; Female genital diseases; Glycogen - metabolism; Gynecology. Andrology. Obstetrics; HIV Infections - complications; HIV Infections - metabolism; Human viral diseases; Humans; Hydrogen Bonding; Infectious diseases; Medical sciences; Middle Aged; Phosphorus - metabolism; Precancerous Conditions - complications; Precancerous Conditions - diagnosis; Precancerous Conditions - metabolism; Spectroscopy, Fourier Transform Infrared; Tumors; Uterine Cervical Neoplasms - complications; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - metabolism; Vaginal Smears; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids
• ISSN: 0022-2143; 1532-6543
• DOI: 10.1016/S0022-2143(00)70017-5

Patients with HIV have an increased incidence of cervical cancer, necessitating increased surveillance. Infrared spectroscopy (IRS) has the potential of aiding the diagnosis of cervical neoplasia and also of providing clues into its pathogenesis. We studied by IRS cervical scrapings from 22 HIV-infected and 23 control women; 8 of the former and none of the latter had dysplasia. The infrared spectra followed three patterns, designated pattern I (similar to that previously associated with normal cervical samples), pattern II (intermediate between patterns I and III), and pattern III (associated with cervical neoplasia). Compared with HIV-negative controls, HIV-infected women had a higher prevalence of pattern III and a lower prevalence of pattern II; these differences were statistically significant ( P = .015 by χ 2 analysis). Similar spectroscopic changes were present even when only the cytologically normal samples from HIV-positive and HIV-negative women were analyzed. We speculate that these changes may reflect early structural changes associated with cervical neoplasia that are not detectable cytologically. The infrared spectra in the region 950 to 1300 cm –1 could not differentiate cervical samples from HIV-infected and uninfected patients. The potential practical applications of IRS in HIV cervical disease are discussed. (J Lab Clin Med 2000;135:26-31)