Detection of genetic alterations in advanced prostate cancer by comparative genomic hybridization

• Published in: Cancer genetics and cytogenetics 2002-08, Vol.137 (1), p.59-63
• Main Authors: Kasahara, Kotaro, Taguchi, Takahiro, Yamasaki, Ichiro, Kamada, Masayuki, Yuri, Kazunari, Shuin, Taro
• Format: Article
• Language: English
• Subjects: Aged; Biological and medical sciences; Chromosome Aberrations; Chromosome Mapping; Genetic Markers; Humans; Loss of Heterozygosity; Male; Medical sciences; Middle Aged; Neoplasm Staging; Nephrology. Urinary tract diseases; Nucleic Acid Hybridization; Prostatic Neoplasms - genetics; Prostatic Neoplasms - pathology; Tumors of the urinary system; Urinary tract. Prostate gland
• ISSN: 0165-4608; 1873-4456
• DOI: 10.1016/S0165-4608(02)00552-6

In this study, we examined nine cases of advanced Japanese prostate cancer by comparative genomic hybridization (CGH) to detect chromosomal imbalances across the entire genome and to identify several new regions likely to contain genes important to the development and progression of this disease. These cases had been previously examined for numerical chromosomal aberrations by fluorescence in situ hybridization (FISH). By CGH, the following regions were found to be over-represented (gains), with fluorescence ratio values higher than the threshold: 4p, 6p, 8q, 11q, 12q, 15q, 16p, 17q, 20, and 21 (>4 cases); underrepresentation (losses) involved: 1q, 4q, 5q, 6q, 13q, 14q, and 22 (>4 cases). The shortest regions of overlap (SRO) of gains were noted at 8q24.1∼q24.3, 12q23, and 17q23∼q24 (>5 cases). The SRO of losses were seen at 5q14∼q21, 6q16.1∼q21, 13q21.3∼q22, and 14q21 (>5 cases). Notably, the gain of chromosomes 8 and 12 by CGH was in agreement with the FISH data, suggesting that the gain of chromosomes 8 and 12 may play an important role in prostate carcinogenesis. The genes on the SRO regions were also discussed in relation to oncogenes and bone metastases.