Cholera and pertussis toxins inhibit differently hypothermic and anti-opioid effects of neuropeptide FF

In mice pretreated intracerebroventricularly (i.c.v.) with pertussis or cholera toxins, effects of neuropeptide FF (NPFF), on hypothermia and morphine-induced analgesia, were assessed. NPFF and a potent NPFF agonist, 1DMe (0.005–22 nmol) injected into the lateral ventricle decreased morphine analges...

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Bibliographic Details
Published in:Regulatory peptides 2001-04, Vol.98 (1), p.13-18
Main Authors: Francés, Bernard, Lahlou, Hicham, Zajac, Jean-Marie
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cholera toxin
Cholera Toxin - pharmacology
Fundamental and applied biological sciences. Psychology
Hypothermia
Hypothermia, Induced
Male
Mice
Morphine - pharmacology
Morphine analgesia
Narcotic Antagonists - pharmacology
Narcotics - pharmacology
Neuropeptide FF
Oligopeptides - pharmacology
Pain Measurement - drug effects
Pertussis Toxin
Receptors, Neuropeptide - drug effects
Reflex - drug effects
Somesthesis and somesthetic pathways (proprioception, exteroception, nociception)
interoception
electrolocation. Sensory receptors
Tail
Vertebrates: nervous system and sense organs
Virulence Factors, Bordetella - pharmacology
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Summary:In mice pretreated intracerebroventricularly (i.c.v.) with pertussis or cholera toxins, effects of neuropeptide FF (NPFF), on hypothermia and morphine-induced analgesia, were assessed. NPFF and a potent NPFF agonist, 1DMe (0.005–22 nmol) injected into the lateral ventricle decreased morphine analgesia and produced naloxone (2.5 mg kg −1, s.c.)-resistant hypothermia after administration into the third ventricle. Cholera toxin (CTX 1 μg, i.c.v.) pretreatment (24 or 96 h before) inhibited the effect of 1DMe on body temperature, but failed to reverse its anti-opioid activity in the tail-flick test. CTX reduced hypothermia induced by a high dose of morphine (8 nmol, i.c.v.) but not the analgesic effect due to 3 nmol morphine. Pertussis toxin (PTX) pretreatment inhibited both morphine-hypothermia and -analgesia but did not modify hypothermia induced by 1DMe. The present results suggest that NPFF-induced hypothermia depends on the stimulation of Gs (but not Gi) proteins. In contrast, anti-opioid effects resulting from NPFF-receptor stimulation do not involve a cholera toxin-sensitive transducer protein.
ISSN:0167-0115
1873-1686
DOI:10.1016/S0167-0115(00)00188-9