Bio-LDH nanohybrid for gene therapy

Nano-sized inorganic clay, such as layered double hydroxide (LDH), has been demonstrated as delivery carrier for genes and drugs by hybridizing with DNA and c-antisense oligonucleotide (As- myc). Upon intercalating biomolecules into hydroxide layers, the basal spacing of LDH increases from 8.7 Å (fo...

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Bibliographic Details
Published in:Solid state ionics 2002-11, Vol.151 (1), p.229-234
Main Authors: Kwak, Seo-Young, Jeong, Yong-Joo, Park, Jong-Sang, Choy, Jin-Ho
Format: Article
Language:English
Subjects:
Anionic clays
Bioinorganic nanohybrid
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Gene therapy
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Layered compounds
Nonviral vector
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Summary:Nano-sized inorganic clay, such as layered double hydroxide (LDH), has been demonstrated as delivery carrier for genes and drugs by hybridizing with DNA and c-antisense oligonucleotide (As- myc). Upon intercalating biomolecules into hydroxide layers, the basal spacing of LDH increases from 8.7 Å (for NO 3 −) to 23.9 Å (DNA) and 17.1 Å (As- myc), respectively. A strong suppression of cell growth (65%) is observed when the HL-60 cells are incubated with 20 μM As- myc–LDH hybrid. However, LDH itself is found to be noncytotoxic on HL-60 cells (leukemia cells). Based on these findings, it is proved that LDHs can act as a new inorganic carrier which is completely different from ever existing nonviral vectors in terms of its chemical bonding and structure.
ISSN:0167-2738
1872-7689
DOI:10.1016/S0167-2738(02)00714-2