Superoxide anion scavenger properties of sparteine, a quinolizidine alkaloid from Lupinus

Peroxidase activity in lupin ( Lupinus albus cv. Multolupa) hypocotyls is located, as are quinolizidine-type alkaloids, in epidermal tissues. Since these alkaloids have been shown to suffer a rapid turnover and degradation in lupin tissues, the ability of sparteine (a typical tetracyclic quinolizidi...

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Bibliographic Details
Published in:Journal of plant physiology 1997, Vol.150 (1), p.5-8
Main Authors: de Pinto, M.C., Ros Barceló, A.
Format: Article
Language:English
Subjects:
ALCALOIDE
ALCALOIDES
ALKALOIDS
Biological and medical sciences
CYTISIN
ENZIMAS
ENZYME
ENZYMES
EPIDERME
EPIDERMIS
ESPECTROMETRIA
Fundamental and applied biological sciences. Psychology
HIPOCOTILOS
HYPOCOTYLE
HYPOCOTYLS
LEGUMINOSAE
Leguminoseae
LUPINUS ALBUS
Metabolism
Metabolism. Physicochemical requirements
PEROXIDASAS
peroxidase
PEROXIDASES
PEROXIDES
PEROXIDOS
PEROXYDASE
PEROXYDE
Plant physiology and development
QUINOLIZIDINALKALOID
quinolizidine-type alkaloids
SPARTEIN
sparteine
SPECTROMETRIE
SPECTROMETRY
SUPEROXID
SUPEROXIDE DISMUTASE
superoxide scavenger properties
SUPEROXIDO DISMUTASA
SUPEROXYDE DISMUTASE
WEISSLUPINE
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Description
Summary:Peroxidase activity in lupin ( Lupinus albus cv. Multolupa) hypocotyls is located, as are quinolizidine-type alkaloids, in epidermal tissues. Since these alkaloids have been shown to suffer a rapid turnover and degradation in lupin tissues, the ability of sparteine (a typical tetracyclic quinolizidine alkaloid) and cytisine (a typical tricyclic α-pyridone quinolizidine alkaloid) to act as peroxidase substrates was studied. However, neither alkaloid was oxidized by a partially purified lupin peroxidase fraction, or by horseradish peroxidase, as was ascertained by spectrophotometric and GC-MS studies. Likewise, these quinolizidine-type alkaloids were not substrates of a laccase of fungal origin. However, sparteine, unlike cytisine, acted as a superoxide scavenger, when the superoxide anions were generated in a phenazin methosulphate-NADH system and determined by the superoxide dismutase-sensitive reduction of nitroblue tetrazolium. The IC 50 value was 2.0 mmol/L. These results are discussed in the light of a possible protective role against oxidative damage of sparteine in Lupinus.
ISSN:0176-1617
1618-1328
DOI:10.1016/S0176-1617(97)80173-3