Quantitative reduction of type I adenylyl cyclase in human alcoholics

The amounts of adenylyl cyclase type I (AC I) were examined in various parts of the postmortem brains from alcoholics who prior to death had been abstinent from alcohol for at least 6 months and compared with controls using immunoblot analysis with anti-AC I specific antibody. It was revealed that a...

Full description

Saved in:
Bibliographic Details
Published in:Biochimica et biophysica acta 1999-05, Vol.1454 (1), p.11-18
Main Authors: Sohma, Hitoshi, Hashimoto, Eri, Shirasaka, Tomonobu, Tsunematsu, Rieko, Ozawa, Hiroki, Boissl, Karl W., Böning, Jobst, Riederer, Peter, Saito, Toshikazu
Format: Article
Language:English
Subjects:
Adenylyl cyclase type I
Adenylyl cyclase type VIII
Adenylyl Cyclases - analysis
Adenylyl Cyclases - genetics
Adult
Aged
Alcoholic
Alcoholism - blood
Alcoholism - enzymology
Blood Cells - enzymology
Brain - enzymology
Down-Regulation
Humans
Immunoblotting
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction
RFPCR
RNA, Messenger - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The amounts of adenylyl cyclase type I (AC I) were examined in various parts of the postmortem brains from alcoholics who prior to death had been abstinent from alcohol for at least 6 months and compared with controls using immunoblot analysis with anti-AC I specific antibody. It was revealed that a significant reduction of AC I was observed in both frontal and temporal cortices. On the other hand, in other areas (occipital cortex, caudate nucleus, putamen, and hippocampus) the amounts were comparable between alcoholics and controls. In the next step, we examined two subtypes of human AC mRNA levels (AC I and AC VIII) in blood cells by quantitative RT-PCR using [α- 32P]dCTP with two sets of the synthetic oligonucleotide primers based on the DNA sequences reported elsewhere (Villacres, E.C. et al., Genomics 16 (1993) 473–478; J. Parma et al., Biochem. Biophys. Res. Commun. 179 (1991) 455–462). The amounts of amplified DNAs of both AC I and AC VIII were significantly smaller in alcoholics than in controls. On the other hand, the amounts of amplified DNA of β-actin DNA were almost equal between alcoholics and controls. It appears from these results that a reduction in the amount of AC subtypes may be a biological marker for alcoholics.
ISSN:0925-4439
0006-3002
1879-260X
DOI:10.1016/S0925-4439(99)00018-6