Oxidation of phenolic compounds from Aloe barbadensis by peroxidase activity: Possible involvement in defence reactions

Sephadex LH-20 chromatography and reverse phase-high performance liquid chromatography (RP-HPLC) have been combined to analyse different phenolics in Aloe barbadensis Mill. Among them, a new chromone peak was found. Whole phenolics, and anthrone and chromone fractions were assayed as substrates of e...

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Bibliographic Details
Published in:Plant physiology and biochemistry 2001-06, Vol.39 (6), p.521-527
Main Authors: Esteban-Carrasco, Alberto, López-Serrano, Matías, Zapata, José Miguel, Sabater, Bartolomé, Martín, Mercedes
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Agronomy. Soil science and plant productions
ALOE BARBADENSIS
Biological and medical sciences
CATECHOL OXIDASE
CATECHOL OXYDASE
CATECOL OXIDASA
COMPOSE PHENOLIQUE
COMPUESTOS FENOLICOS
DEFENCE MECHANISMS
defence reaction
DISEASE RESISTANCE
DRUG PLANTS
Economic plant physiology
ENZYME ACTIVITY
Fundamental and applied biological sciences. Psychology
MECANISME DE DEFENSE
MECANISMOS DE DEFENSA
Metabolism
Metabolism. Physicochemical requirements
Nitrogen metabolism and other ones (excepting carbon metabolism)
Nutrition. Photosynthesis. Respiration. Metabolism
peroxidase
PHENOLIC COMPOUNDS
Plant physiology and development
PLANTAS MEDICINALES
PLANTE MEDICINALE
polyphenol oxidase
reactive oxygen species
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
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Summary:Sephadex LH-20 chromatography and reverse phase-high performance liquid chromatography (RP-HPLC) have been combined to analyse different phenolics in Aloe barbadensis Mill. Among them, a new chromone peak was found. Whole phenolics, and anthrone and chromone fractions were assayed as substrates of endogenous peroxidases (donor:hydrogen-peroxide oxidoreductase; EC 1.11.1.7) and polyphenol oxidases (1,2-benzenediol:oxygen oxidoreductase; EC 1.10.3.1) by following the disappearance of specific RP-HPLC peaks after appropriate incubations in the presence and in absence of H 2O 2. Chromones, but not anthrones, were found to be good substrates of peroxidases. It is postulated that peroxidase oxidation of chromones may have a protective and sealing effect against infection after wounding. Polyphenol oxidases may have a secondary, if any, effect.
ISSN:0981-9428
1873-2690
DOI:10.1016/S0981-9428(01)01270-0