Mutation of p53 gene codon 63 in saliva as a molecular marker for oral squamous cell carcinomas

The inactivation of tumor suppressor gene (TSG) is important during multistage carcinogenesis. The p53 TSG is frequently mutated in oral squamous cell carcinomas. These mutations can serve as very specific markers for the presence of tumor cells in a background of normal cells. In this study, 10 ora...

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Bibliographic Details
Published in:Oral oncology 2000-05, Vol.36 (3), p.272-276
Main Authors: Liao, Pao-Hsin, Chang, Yu-Chao, Huang, Ming-Fa, Tai, Kuo-Wei, Chou, Ming-Yung
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Carcinoma, Squamous Cell - diagnosis
Carcinoma, Squamous Cell - genetics
Case-Control Studies
Chi-Square Distribution
Codon
DNA sequencing
Fundamental and applied biological sciences. Psychology
General aspects. Genetic counseling
Genes, p53 - genetics
Genes. Genome
Genetic Markers
Humans
Male
Medical genetics
Medical sciences
Middle Aged
Molecular and cellular biology
Molecular genetics
Mouth Neoplasms - diagnosis
Mouth Neoplasms - genetics
Oral squamous cell carcinoma
p53
Polymerase Chain Reaction - methods
Saliva
Saliva - chemistry
Sequence Analysis, DNA
Tropical medicine
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Summary:The inactivation of tumor suppressor gene (TSG) is important during multistage carcinogenesis. The p53 TSG is frequently mutated in oral squamous cell carcinomas. These mutations can serve as very specific markers for the presence of tumor cells in a background of normal cells. In this study, 10 oral squamous cell carcinoma patients and 27 normal dental students were collected from Chung Shan Medical and Dental College Hospital, Taichung, Taiwan. Extractions of DNA from saliva were obtained. Exon 4 and intron 6 within the p53 gene were amplified with polymerase chain reactions (PCRs) followed by DNA sequence analysis. DNA sequence analysis of PCR products revealed that five of eight (62.5%) tumor saliva samples and five of 27 (18.52%) healthy saliva samples contained p53 exon 4 codon 63 mutations. These results were significantly different by using Chi-square test ( P
ISSN:1368-8375
1879-0593
DOI:10.1016/S1368-8375(00)00005-1