Combined effects of the plant growth-promoting bacterium Pseudomonas putida UW4 and salinity stress on the Brassica napus proteome

• Published in: Applied soil ecology : a section of Agriculture, ecosystems & environment ecosystems & environment, 2012-10, Vol.61, p.255-263
• Main Authors: Cheng, Zhenyu, Woody, Owen Z., McConkey, Brendan J., Glick, Bernard R.
• Format: Article
• Language: English
• Subjects: 1-Aminocyclopropane-1-carboxylic acid; Agronomy. Soil science and plant productions; Biochemistry and biology; Biological and medical sciences; Brassica napus; canola; Chemical, physicochemical, biochemical and biological properties; Ethylene; Fundamental and applied biological sciences. Psychology; host plants; mass spectrometry; Microbiology; photosynthesis; Physics, chemistry, biochemistry and biology of agricultural and forest soils; Phytoremediation; plant growth-promoting rhizobacteria; plant proteins; protein synthesis; proteome; Proteomics; Pseudomonas putida; Salt; salt stress; salts; shoots; single cell protein; Soil bacteria; Soil science; stress response; two-dimensional gel electrophoresis
• ISSN: 0929-1393; 1873-0272
• DOI: 10.1016/j.apsoil.2011.10.006

► Bacterial ACC deaminase alleviates salinity stress on plants. ► Proteomic investigations revealed the individual and combined effects of bacterium and salinity stress on plant. ► The impacts of bacterial ACC deaminase on these effects on plant proteome have also been examined. The influences of two strains of Pseudomonas putida UW4, a plant growth-promoting bacterium (PGPB) on the shoot and root proteomic profiles of Brassica napus (canola) were examined under salinity stress using two-dimensional difference gel electrophoresis. The two strains (wild-type UW4 and an AcdS minus mutant strain) differ in the availability of a functional 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, a bacterial protein known to play a role in host plant's stress responses. In total, ninety protein spots (representing 76 different proteins) with significantly altered expression levels in the presence of salt and/or bacteria were identified by mass spectrometry. Many of the identified proteins are involved in photosynthesis, anti-oxidative processes, transportation across membranes, and pathogenesis-related responses. The effects of salt and bacteria on the canola proteome were shown to be quite diverse, with salinity stress causing more dramatic plant protein expression changes than bacteria. In addition, bacteria were demonstrated to moderate some of the salt effects on plant protein differential expression. This work contributes to the understanding of how plant protein expression is affected by various environmental signals.