True lipase activity and in vitro digestibility of potential lipid sources for the spiny lobster Panulirus argus feeds

• Published in: Aquaculture 2022-06, Vol.555, p.738191, Article 738191
• Main Authors: Rodríguez-Viera, Leandro, Perera, Erick, Agredano Pila, IsabelM, Moyano, Francisco J., Mancera, Juan M., Díaz, Manuel
• Format: Article
• Language: English
• Subjects: Crustaceans; Feed oils; In vitro digestibility; Lipase; Lipids; Lipolytic activity; Lobster; pH-stat
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2022.738191

Spiny lobster aquaculture has increased during the past decades, attended to the lobster high demand and commercial value. However, the absence of cost-effective and nutritionally adequate formulated feeds remains as one of the drawbacks for the sustainable expansion of this activity. Despite lipids are important nutrients and energy sources for these crustaceans, lipid digestion remains poorly understood. In this study, the pH-Stat method was used for studying in vitro the lipolytic activity in the digestive gland of the spiny lobster Panulirus argus and the digestibility of 18 oils from animal, plant and microalgae origin. We complemented these analyses by evaluating the effects of several emulsifiers (non-ionic surfactants and phospholipids) on lipid digestion, and by making a partial characterization of enzymes with lipolytic activity in this species. Results demonstrate for the first time the presence of true digestive lipases in spiny lobsters, and also that P. argus has the ability to efficiently hydrolyzed both animal and vegetable oils. Fish oils are highly digestible but algae oils and plant derived oils such as rapeseed oil, should be also taken into account in feed formulation for P. argus. In addition, results indicate that hydrolyzed soy lecithin presents the greatest potential to be used as an emulsifier ingredient in lobsters' feeds, for which optimal inclusion level in feed needs to be determined. This study increases our understanding on lipid digestion in lobsters and may assist in selecting appropriated oils and emulsifiers for P. argus feeds. •“True lipases” in spiny lobsters•Emulsifier ingredient in lobsters' feeds•Highly digestible oils for lobsters' feeds•Viable oils nutritional option for crustaceans diets