Ca2+ regulation of mitochondrial function in neurons

• Published in: Biochimica et biophysica acta. Bioenergetics 2014-10, Vol.1837 (10), p.1617-1624
• Main Authors: Rueda, Carlos B., Llorente-Folch, Irene, Amigo, Ignacio, Contreras, Laura, González-Sánchez, Paloma, Martínez-Valero, Paula, Juaristi, Inés, Pardo, Beatriz, del Arco, Araceli, Satrústegui, Jorgina
• Format: Article
• Language: English
• Subjects: Aspartate–glutamate transporter; ATP-Mg/Pi transporter; Calcium; Calcium-regulated transport; Mitochondrion; Neuronal respiration
• ISSN: 0005-2728; 1879-2650
• DOI: 10.1016/j.bbabio.2014.04.010

Calcium is thought to regulate respiration but it is unclear whether this is dependent on the increase in ATP demand caused by any Ca2+ signal or to Ca2+ itself. [Na+]i, [Ca2+]i and [ATP]i dynamics in intact neurons exposed to different workloads in the absence and presence of Ca2+ clearly showed that Ca2+-stimulation of coupled respiration is required to maintain [ATP]i levels. Ca2+ may regulate respiration by activating metabolite transport in mitochondria from outer face of the inner mitochondrial membrane, or after Ca2+ entry in mitochondria through the calcium uniporter (MCU). Two Ca2+-regulated mitochondrial metabolite transporters are expressed in neurons, the aspartate–glutamate exchanger ARALAR/AGC1/Slc25a12, a component of the malate–aspartate shuttle, and the ATP-Mg/Pi exchanger SCaMC-3/APC2/Slc25a23, with S0.5 for Ca2+ of 300nM and 3.4μM, respectively. The lack of SCaMC-3 results in a smaller Ca2+-dependent stimulation of respiration only at high workloads, as caused by veratridine, whereas the lack of ARALAR reduced by 46% basal OCR in intact neurons using glucose as energy source and the Ca2+-dependent responses to all workloads: a reduction of about 65–70% in the response to the high workload imposed by veratridine, and completely suppression of the OCR responses to moderate (K+-depolarization) and small (carbachol) workloads, effects reverted by pyruvate supply. For K+-depolarization, this occurs in spite of the presence of large [Ca2+]mit signals and increased formation of mitochondrial NAD(P)H. These results show that ARALAR-MAS is a major contributor of Ca2+-stimulated respiration in neurons by providing increased pyruvate supply to mitochondria. In its absence and under moderate workloads, matrix Ca2+ is unable to stimulate pyruvate metabolism and entry in mitochondria suggesting a limited role of MCU in these conditions. This article was invited for a Special Issue entitled: 18th European Bioenergetic Conference. [Display omitted] •Ca2+ upregulates workload-dependent respiration regardless of Ca2+ induced ATP demand.•Ca2+ stimulation of respiration is required to maintain cytosolic ATP levels.•ARALAR-MAS mediated pyruvate supply is required at all workloads.•SCaMC-3 mediated AdN supply increases respiratory stimulation at high workloads.•Matrix Ca2+ increase is not sufficient for full respiratory stimulation in ARALAR KO.