Ab initio electron propagator calculations of transverse conduction through DNA nucleotide bases in 1-nm nanopore corroborate third generation sequencing

• Published in: Biochimica et biophysica acta 2016-01, Vol.1860 (1), p.140-145
• Main Authors: Kletsov, Aleksey A., Glukhovskoy, Evgeny G., Chumakov, Aleksey S., Ortiz, Joseph V.
• Format: Article
• Language: English
• Subjects: DNA - chemistry; DNA transverse conduction; Electric Conductivity; Electron propagator theory; Electron Transport; Nanopores; Nucleobase conductance; Nucleotide identification; Onset voltage; Sequence Analysis, DNA; Third generation sequencing
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2015.10.013

The conduction properties of DNA molecule, particularly its transverse conductance (electron transfer through nucleotide bridges), represent a point of interest for DNA chemistry community, especially for DNA sequencing. However, there is no fully developed first-principles theory for molecular conductance and current that allows one to analyze the transverse flow of electrical charge through a nucleotide base. We theoretically investigate the transverse electron transport through all four DNA nucleotide bases by implementing an unbiased ab initio theoretical approach, namely, the electron propagator theory. The electrical conductance and current through DNA nucleobases (guanine [G], cytosine [C], adenine [A] and thymine [T]) inserted into a model 1-nm Ag–Ag nanogap are calculated. The magnitudes of the calculated conductance and current are ordered in the following hierarchies: gA>gG>gC>gT and IG>IA>IT>IC correspondingly. The new distinguishing parameter for the nucleobase identification is proposed, namely, the onset bias magnitude. Nucleobases exhibit the following hierarchy with respect to this parameter: Vonset(A)