Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches

Cell wall proteins (CWPs) are important both for maintenance of cell structure and for responses to abiotic and biotic stresses. In this study, a destructive CWP purification procedure was adopted using wheat seedling roots and the purity of the CWP extract was confirmed by minimizing the activity o...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2010-01, Vol.1804 (1), p.124-136
Main Authors: Kong, Fan-Jiang, Oyanagi, Atsushi, Komatsu, Setsuko
Format: Article
Language:English
Subjects:
Cell wall
Cell Wall - chemistry
Chromatography, Liquid
Down-Regulation
Flooding stress
Floods
LC-MS/MS
Nanotechnology
Plant Proteins - chemistry
Plant Proteins - isolation & purification
Plant Roots - genetics
Proteome - analysis
Proteomics
Proteomics - methods
Seedlings - growth & development
Stress, Physiological
Tandem Mass Spectrometry
Triticum - genetics
Triticum - growth & development
Wheat
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Summary:Cell wall proteins (CWPs) are important both for maintenance of cell structure and for responses to abiotic and biotic stresses. In this study, a destructive CWP purification procedure was adopted using wheat seedling roots and the purity of the CWP extract was confirmed by minimizing the activity of glucose-6-phosphate dehydrogenase, a cytoplasmic marker enzyme. To determine differentially expressed CWPs under flooding stress, gel-based proteomic and LC-MS/MS-based proteomic techniques were applied. Eighteen proteins were found to be significantly regulated in response to flood by gel-based proteomics and 15 proteins by LC MS/MS-based proteomics. Among the flooding down-regulated proteins, most were related to the glycolysis pathway and cell wall structure and modification. However, the most highly up-regulated proteins in response to flooding belong to the category of defense and disease response proteins. Among these differentially expressed proteins, only methionine synthase, β-1,3-glucanases, and β-glucosidase were consistently identified by both techniques. The down-regulation of these three proteins suggested that wheat seedlings respond to flooding stress by restricting cell growth to avoid energy consumption; by coordinating methionine assimilation and cell wall hydrolysis, CWPs played critical roles in flooding responsiveness.
ISSN:1570-9639
0006-3002
1878-1454
DOI:10.1016/j.bbapap.2009.09.023