Investigating the allosteric reverse signalling of PARP inhibitors with microsecond molecular dynamic simulations and fluorescence anisotropy

• Published in: Biochimica et biophysica acta 2014-10, Vol.1844 (10), p.1765-1772
• Main Authors: Marchand, Jean-Rémy, Carotti, Andrea, Passeri, Daniela, Filipponi, Paolo, Liscio, Paride, Camaioni, Emidio, Pellicciari, Roberto, Gioiello, Antimo, Macchiarulo, Antonio
• Format: Article
• Language: English
• Subjects: Cancer; Fluorescence anisotropy; Molecular dynamics; Neuroprotection; PARP; Polypharmacology
• ISSN: 1570-9639; 0006-3002; 1878-1454
• DOI: 10.1016/j.bbapap.2014.07.012

The inhibition of the poly(ADP-ribose) polymerase (PARP) family members is a strategy pursued for the development of novel therapeutic agents in a range of diseases, including stroke, cardiac ischemia, cancer, inflammation and diabetes. Even though some PARP-1 inhibitors have advanced to clinical setting for cancer therapy, a great deal of attention is being devoted to understand the polypharmacology of current PARP inhibitors. Besides blocking the catalytic activity, recent works have shown that some PARP inhibitors exhibit a poisoning activity, by trapping the enzyme at damaged sites of DNA and forming cytotoxic complexes. In this study we have used microsecond molecular dynamics to study the allosteric reverse signalling that is at the basis of such an effect. We show that Olaparib, but not Veliparib and HYDAMTIQ, is able to induce a specific conformational drift of the WGR domain of PARP-1, which stabilizes PARP-1/DNA complex through the locking of several salt bridge interactions. Fluorescence anisotropy assays support such a mechanism, providing the first experimental evidence that HYDAMTIQ, a potent PARP inhibitor with neuroprotective properties, is less potent than Olaparib to trap PARP-1/DNA complex. [Display omitted] •MDs of hPARP-1 with inhibitors and DNA show different allosteric reverse signalling.•Olaparib stabilizes the complex favouring salt bridge interactions in the enzyme.•HYDAMTIQ induces conformational changes that do not favour salt bridge interactions.•Fluorescence anisotropy supports the results of MD studies.