Defining mass transfer in a capillary wave micro-bioreactor for dose-response and other cell-based assays

• Published in: Biochemical engineering journal 2020-09, Vol.161, p.107667, Article 107667
• Main Authors: Frey, Lasse Jannis, Vorländer, David, Rasch, Detlev, Meinen, Sven, Müller, Bernhard, Mayr, Torsten, Dietzel, Andreas, Grosch, Jan-Hendrik, Krull, Rainer
• Format: Article
• Language: English
• Subjects: Capillary waves; Droplets; kLa; Micro-bioreactor; Mixing; Oscillation
• ISSN: 1369-703X
• DOI: 10.1016/j.bej.2020.107667

•Capillary waves induced by vertical oscillation are used to mix a sessile droplet micro-bioreactor.•Oscillations in resonance lead to specific mode patterns on liquid surface.•Mass transfer inside an oscillated liquid droplet is particularly affected by the excitation frequency.•Mixing times and kLa-values can be tailored by oscillation settings. For high-throughput cell culture and associated analytics, droplet-based cultivation systems open up the opportunities for parallelization and rapid data generation. In contrast to microfluidics with continuous flow, sessile droplet approaches enhance the flexibility for fluid manipulation with less operational effort. Generating biologically favorable conditions and promoting cell growth in a droplet, however, is particularly challenging due to mass transfer limitations, which has to be solved by implementing an effective mixing technique. Here, capillary waves induced by vertical oscillation are used to mix inside a sessile droplet micro-bioreactor (MBR) avoiding additional moving parts inside the fluid. Depending on the excitation frequency, different patterns are formed on the oscillating liquid surface, which are described by a model of a vibrated sessile droplet. Analyzing mixing times and oxygen transport into the liquid, a strong dependency of mass transfer on the oscillation parameters, especially the excitation frequency, is demonstrated. Oscillations at distinct capillary wave resonant frequencies lead to rapid homogenization with mixing times of 2 s and volumetric liquid-phase mass transfer coefficients of more than 340 h−1. This shows that the mass transfer in a droplet MBR can be specifically controlled via capillary waves, what is subsequently demonstrated for cultivations of Escherichia coli BL21 cells. Therefore, the presented MBR with vertical oscillation mixing is a promising analytical tool to perform cellular assays and experiments in parallel with intensified and tailored mass transfer.