Evaluation of dynamic binding performance of C-phycocyanin and allophycocyanin in Spirulina platensis algae by aminated polyacrylonitrile nanofiber membrane

• Published in: Biochemical engineering journal 2020-09, Vol.161, p.107686, Article 107686
• Main Authors: Liu, Bing-Lan, Chai, Wai Siong, Show, Pau Loke, Chen, Jia-Yi, Chang, Yu-Kaung
• Format: Article
• Language: English
• Subjects: Allophycocyanin; Binding performance; C-phycocyanin; Polyamine nanofiber membrane; Spirulina platensis
• ISSN: 1369-703X
• DOI: 10.1016/j.bej.2020.107686

[Display omitted] •The order of dynamic binding efficiency for CPC and APC is P-EDA > P-CN > P-HDA.•The contaminating proteins can be removed during the adsorption and wash procedures.•A highly effective capture of APC/CPC can be achieved in membrane chromatography.•APC and CPC nanofiber membranes can act as an antibacterial & antioxidant membranes. Polyacrylonitrile nanofibrous membrane (PAN) was prepared by electrospinning technique and was directly converted to polyamine membrane by 50 % and 100 % (w/w) ethylenediamine (EDA) and hexamethylenediamine (HDA), respectively. The aminated PAN nanofiber membrane equipped with protein liquid chromatography were used to evaluate the dynamic binding capacity for C-phycocyanin (CPC) and allophycocyanin (APC) from disrupted Spirulina platensis under different operating conditions. Experimental results showed that the contaminating proteins were completely removed during the flow and wash procedures. However, a large amount of APC and CPC can be retained on the membranes. Consequently, the aminated PAN membrane may have a high potential for use in the separation of APC/CPC and TP from a complex algae feedstock. In this study, a highly effective capture of APC/CPC can be achieved in one-step membrane chromatography, which can be directly employed as an antibacterial and antioxidant nanofiber membranes.