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Optimization of the in-vitro refolding of biotherapeutic Fab Ranibizumab

Recombinant biotherapeutics expressed as inclusion bodies require solubilization and subsequent refolding to attain a functionally active state. But the refolding step often acts as a bottleneck due to a low throughput, cumbersome and expensive framework, especially in the case of complex, multi-dom...

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Bibliographic Details
Published in:Biochemical engineering journal 2022-11, Vol.187, p.108601, Article 108601
Main Authors: Sharma, Rashmi, Anupa, Anupa, Kateja, Nikhil, Rathore, Anurag S.
Format: Article
Language:English
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Summary:Recombinant biotherapeutics expressed as inclusion bodies require solubilization and subsequent refolding to attain a functionally active state. But the refolding step often acts as a bottleneck due to a low throughput, cumbersome and expensive framework, especially in the case of complex, multi-domain proteins such as antibody fragment (Fab) molecules. Fab Ranibizumab, with two domains and five disulfide bonds, currently presents a daunting task to be refold. In this paper, the in-vitro refolding of this Fab over 24 h was investigated, using two different methodologies. The first protocol employed the traditional two-step DoE for screening and optimization. The entire refolding process was considered as a single process step. The second approach derived inferences from the data of analytical tools like intrinsic fluorescence, zeta potential and RP-HPLC to highlight a possible time-based molecular behavior during refolding. This led to the identification of a breakpoint at the 8th hour of the process, proposing initial occurrences of the native tertiary conformation. Based on this observation, segmented DoEs were conducted to optimize the two time zones of the refolding process (0–8 h and 8–24 h). This unconventional segment-based optimization approach led to a 55 % increment over the standard conventional optimization methodology, performed for the same Fab, Ranibizumab. It prevailed with an effective yield of 32 % over the conventional strategy with 20.6 %. •Analyzing the in vitro refolding of Ranibizumab using Process Analytical Technology tools.•Bisecting the refolding of the Fab for a targeted optimization (unconventional approach).•Comparing the strategy with the conventional DoE based approach (55% yield increment).
ISSN:1369-703X
1873-295X
DOI:10.1016/j.bej.2022.108601