In vivo editing of the pan-endothelium by immunity evading simian adenoviral vector

• Published in: Biomedicine & pharmacotherapy 2023-02, Vol.158, p.114189, Article 114189
• Main Authors: Lorincz, Reka, Alvarez, Aluet Borrego, Walkey, Christopher J., Mendonça, Samir A., Lu, Zhi Hong, Martinez, Alexa E., Ljungberg, Cecilia, Heaney, Jason D., Lagor, William R., Curiel, David T.
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Animals; Capsid Proteins - genetics; CRISPR-Cas Systems - genetics; Endothelium; Gene Editing - methods; Gene editing of vascular endothelium; Genetic Therapy - methods; Genetic Vectors - genetics; Mice; Selective gene delivery; Targeted nonhuman adenoviral vector
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2022.114189

Biological applications deriving from the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 site-specific nuclease system continue to impact and accelerate gene therapy strategies. Safe and effective in vivo co-delivery of the CRISPR/Cas9 system to target somatic cells is essential in the clinical therapeutic context. Both non-viral and viral vector systems have been applied for this delivery matter. Despite elegant proof-of-principle studies, available vector technologies still face challenges that restrict the application of CRISPR/Cas9-facilitated gene therapy. Of note, the mandated co-delivery of the gene-editing components must be accomplished in the potential presence of pre-formed anti-vector immunity. Additionally, methods must be sought to limit the potential of off-target editing. To this end, we have exploited the molecular promiscuities of adenovirus (Ad) to address the key requirements of CRISPR/Cas9-facilitated gene therapy. In this regard, we have endeavored capsid engineering of a simian (chimpanzee) adenovirus isolate 36 (SAd36) to achieve targeted modifications of vector tropism. The SAd36 vector with the myeloid cell-binding peptide (MBP) incorporated in the capsid has allowed selective in vivo modifications of the vascular endothelium. Importantly, vascular endothelium can serve as an effective non-hepatic cellular source of deficient serum factors relevant to several inherited genetic disorders. In addition to allowing for re-directed tropism, capsid engineering of nonhuman primate Ads provide the means to circumvent pre-formed vector immunity. Herein we have generated a SAd36. MBP vector that can serve as a single intravenously administered agent allowing effective and selective in vivo editing for endothelial target cells of the mouse spleen, brain and kidney. The data that support the findings of this study are available from the corresponding author upon reasonable request. [Display omitted]