3D bioprinting of cell-laden carbopol bioinks

Traditional in vitro culture models are unable to fully reflect the organ microenvironment, due to differences in terms of cell morphology, protein expression, cell-cell and cell-matrix interactions, and drug response. In contrast, the flexibility of bioprinting modes allows for the deposition of ce...

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Bibliographic Details
Published in:Bioprinting (Amsterdam, Netherlands) Netherlands), 2021-06, Vol.22, p.e00135, Article e00135
Main Authors: Baruffaldi, Désirée, Pirri, Candido Fabrizio, Frascella, Francesca
Format: Article
Language:English
Subjects:
3D cell culture
Bioink
Carbopol
Extrusion-based bioprinter
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Summary:Traditional in vitro culture models are unable to fully reflect the organ microenvironment, due to differences in terms of cell morphology, protein expression, cell-cell and cell-matrix interactions, and drug response. In contrast, the flexibility of bioprinting modes allows for the deposition of cell-containing biomaterials in any free-form-inspired 3D structures on chip. The main purpose of this study was to design and optimize commercially available Carbopol-based 3D printing formulations, because of their many advantages, such as low-cost, the ability to produce clear and stable gels, and the water thickening. For this purpose, three different Carbopol gels (EDT 2020 NF, Ultrez 10 NF and NF-980) were tested in terms of printability and biocompatibility, with lung cancer epithelial (A549) and normal lung fibroblast (MRC-5) cells. This study demonstrates that Carbopol is a promising candidate for the 3D printing of cell-laden constructs, both in terms of rheology and printing performance.
ISSN:2405-8866
2405-8866
DOI:10.1016/j.bprint.2021.e00135