Immobilization of hyaluronic acid from Lactococcus lactis on polyethylene terephthalate for improved biocompatibility and drug release

[Display omitted] •Characterized hyaluronic acid from metabolically engineered Lactococcus lactis (HAL) & evaluated its biocompatibility.•Covalently immobilized HAL on hydrolyzed polyethylene terephthalate and characterized the modified surfaces.•HAL immobilization enhanced haemocompatibility &a...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate polymers 2019-02, Vol.206, p.132-140
Main Authors: Ramachandran, Balaji, Chakraborty, Sudip, Kannan, Ramya, Dixit, Madhulika, Muthuvijayan, Vignesh
Format: Article
Language:English
Subjects:
Drug release
Endothelialization
Haemocompatibility
Hyaluronic acid
Polyethylene terephthalate
Surface modification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •Characterized hyaluronic acid from metabolically engineered Lactococcus lactis (HAL) & evaluated its biocompatibility.•Covalently immobilized HAL on hydrolyzed polyethylene terephthalate and characterized the modified surfaces.•HAL immobilization enhanced haemocompatibility & endothelialization.•HAL matrix on the modified surface was used for model drug loading and release. Hyaluronic acid from metabolically engineered Lactococcus lactis (HAL) was characterized for its biocompatibility and immobilized on the polyethylene terephthalate (PET) surface. HAL was chemically crosslinked on hydrolyzed PET (hPET) surface to form HAL-coated PET (hPET-HAL). The unmodified and modified PET were characterized by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), contact angle measurement, thermogravimetric analysis (TGA), universal testing machine (UTM) and assessed for their biocompatibility. FT-IR confirmed the successful immobilization of HAL on the hPET surface. HAL coating significantly improved the haemocompatibility compared to hPET and unmodified PET. Endothelial cell attachment was significantly improved on hPET-HAL and hPET surfaces compared to the unmodified PET. Model drugs (aspirin and methylene blue) were loaded into the HAL matrix, and showed complete release at around 18 h. These results confirm that covalent attachment of HAL matrix on PET surfaces is a promising strategy for developing drug-eluting implants with enhanced haemocompatibility and endothelialization.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2018.10.099