Oxygen plasma-modified polycaprolactone nanofiber membrane activates the biological function in cell adhesion, proliferation, and migration through the phosphorylation of FAK and ERK1/2, enhancing bone regeneration

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Switzerland : 1996), 2024-11, Vol.499, p.156003, Article 156003
• Main Authors: Kim, Deogil, Choi, Hyejong, Lee, Min-Ju, Cho, Woong Jin, Lee, Gun Woo, Seo, Young-Kwon, Arai, Yoshie, Lee, Soo-Hong
• Format: Article
• Language: English
• Subjects: Bone defect; Bone regeneration; Cell-regulatory membrane; Plasma treatment; Polycaprolactone; Surface modification
• ISSN: 1385-8947
• DOI: 10.1016/j.cej.2024.156003

[Display omitted] •p-PCL demonstrated improved hydrophilicity and protein adsorption.•p-PCL altered membrane surfaces while maintaining its mechanical integrity.•p-PCL intensified the behavior of mesenchymal stem cells and osteoblast cells.•Cell behavior was regulated by cellular interaction with the p-PCL membrane.•p-PCL demonstrated enhanced bone regeneration capacity in a bone defect model. Bone defects present significant clinical challenges in orthopedics, orthodontics, and maxillofacial surgeries. Accelerated bone regeneration can be facilitated by incorporating mesenchymal stem cells, but limitations in cell survival and growth remain concerns for complete bone repair. Biomaterial-based membranes have been developed to form biocompatible, degradable, and mechanically stable barriers in guided bone regeneration processes. Polycaprolactone (PCL) is a biodegradable polymer widely used as a bone regenerative material because of its favorable mechanical properties. However, its inherent hydrophobicity limits cell adhesion and proliferation, which are necessary for effective bone regeneration. To address this, we fabricated cell-regulatory PCL nanofiber membranes using plasma treatment to enhance induced pluripotent stem cell-derived mesenchymal stem cells and osteoblast growth. The plasma treatment parameters (gas type, flow rate, power, and exposure time) were optimized to enhance PCL’s surface hydrophilicity and protein adsorption properties while maintaining its mechanical integrity. The optimized oxygen plasma-modified PCL membrane demonstrated notable improvements in cell viability, adhesion, and proliferation. In addition, there was improved migration, regulated by cell surface markers and signaling proteins, of the induced pluripotent stem cell-derived mesenchymal stem cells and osteoblasts. In vivo studies in a rat calvarial defect model showed that the plasma-modified PCL membrane dramatically improved new bone formation, facilitating bone regeneration. These findings highlight the potential of plasma treatment of PCL nanofibers to produce effective cell-regulatory membranes for bone defect reconstruction.