Oxygen isotope fractionation of otoliths formed across the maximum thermal range for somatic growth of the American eel Anguilla rostrata

• Published in: Chemical geology 2022-03, Vol.591, p.120706, Article 120706
• Main Authors: Holden, Courtney V., Kyser, T. Kurtis, Longstaffe, Fred J., Wang, Yuxiang, Casselman, John M.
• Format: Article
• Language: English
• Subjects: Calcium carbonate polymorphs; Geothermometer; Growth rate; Oxygen isotopes; Stable carbon isotopes; Temperature
• ISSN: 0009-2541; 1872-6836
• DOI: 10.1016/j.chemgeo.2021.120706

Stable isotope compositions of otoliths can be used to provide thermal histories of fish. This tool, however, is currently unrefined for catadromous species, such as the American eel Anguilla rostrata, where the otolith is formed in both marine and freshwater environments. We reared 2000 elvers in fresh water for 32 weeks in 10 temperature treatments ranging from 10 to 34 °C, and then measured δ18O and δ13C of otoliths to 1) determine how somatic and otolith growth responded to temperature; 2) evaluate the relationship between water temperature and δ18O and δ13C of otoliths; and 3) develop a species-specific isotopic fractionation equation that isolates the freshwater portion of the otolith. Our results show that eel elvers have a high optimum temperature for somatic growth (27–28 °C). Optimum temperature for otolith growth was slightly higher, suggesting that otolith growth can be decoupled from body growth. The expression for carbonate-water isotopic fractionation of American eel otoliths over the temperature range examined was 1000lnαaragonite-water = 14.30(103 T−1) – 18.651. This equation accurately predicted water temperature (predictive error 0.49 °C) for 180 American eel otoliths obtained from a different controlled rearing study (conducted at 22 and 28 °C). This experiment validated the use of the oxygen and carbon isotope compositions of otoliths to determine the thermal history of American eels in fresh water, thus providing a method that can be applied to wild eels. •The O isotope thermometer from 10 to 34 °C was 1000lnαarag-H2O = 14.30 (103/T) – 18.65.•Otoliths generally became more depleted of 13C as temperature increased.•Eel elvers reared at 28 °C grew to the largest body length, weight, and otolith size.•Somatic growth rate was higher than otolith growth rate except at 10 and 34 °C.