Removal of toxic Co-EDTA complex by a halophilic solar-salt-pan isolate Pseudomonas aeruginosa SPB-1

• Published in: Chemosphere (Oxford) 2014, Vol.95, p.503-510
• Main Authors: PARANEEISWARAN, A, SHUKLA, Sudhir K, SUBBA RAO, T, PRASHANTH, K
• Format: Article
• Language: English
• Subjects: Adsorption; Applied sciences; Biodegradation, Environmental; Biological and medical sciences; Biological treatment of sewage sludges and wastes; Biomass; Biotechnology; Cobalt - metabolism; Cobalt - toxicity; Coordination Complexes - metabolism; Coordination Complexes - toxicity; Edetic Acid - metabolism; Edetic Acid - toxicity; Environment and pollution; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General treatment and storage processes; Hydrogen-Ion Concentration; Industrial applications and implications. Economical aspects; Pollution; Pseudomonas aeruginosa - physiology; Wastes
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2013.09.107

In this study, a promising bioremediation approach was developed to remove [Co(III)-EDTA](-) complex that is generated during the waste management process. Though several studies have been reported on bioremediation of cobalt, the removal of [Co(III)-EDTA](-) complex has not been tested. A [Co(III)-EDTA](-) resistant bacterium, Pseudomonas aeruginosa SPB-1 was isolated from the solar-salt-pan and physical parameters were optimized for its growth. The various studies showed that the removal of [Co(III)-EDTA](-) from the bulk liquid was due to the adsorption of the complex by the biomass. Using absorption/desorption isotherm over a range of pH (1-8), the maximum adsorption of [Co(III)-EDTA](-) was found to be at pH 7.0 and maximum desorption from the biomass occurred at pH 1.0, thus rendering an ion exchange property to P. aeruginosa SPB-1 biomass. P. aeruginosa SPB-1 biomass could be used as bio-resin that showed 80.4±3.27% adsorption capacity up to fourth cycle and the biomass was viable till the ninth cycle with 10.5±7.3% adsorption. Radiation tolerance potential i.e. D10 value for the strain was found to be ~300 Gy, which suggests the potential use of the bacterium in bioremediation of moderately active nuclear waste.