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A combined approach based on matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles and liquid chromatography to determine polyphenols from grape residues

•A TiO2 nanoparticle-assisted MSPD extraction was performed.•Experimental design and optimisation were used to define the extraction conditions.•Grape pomace was richer in catechin and more efficient against free radicals.•Grape fruit by-product featured high rutin content and antibacterial activity...

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Bibliographic Details
Published in:Journal of Chromatography A 2021-05, Vol.1644, p.462128, Article 462128
Main Authors: Gómez-Mejía, Esther, Mikkelsen, Line Hartwig, Rosales-Conrado, Noelia, León-González, María Eugenia, Madrid, Yolanda
Format: Article
Language:English
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Summary:•A TiO2 nanoparticle-assisted MSPD extraction was performed.•Experimental design and optimisation were used to define the extraction conditions.•Grape pomace was richer in catechin and more efficient against free radicals.•Grape fruit by-product featured high rutin content and antibacterial activity.•Wine bioresidue extracts could be exploited as preservatives in the industry. A simple and efficient low-cost matrix solid phase dispersion (MSPD) extraction assisted by TiO2 nanoparticles and diatomaceous earth has been developed for the extraction of phenolic compounds from grape and grape pomace wastes. Experimental conditions for MSPD extraction were optimized by a factorial design and a surface response methodology. The simultaneous identification and quantification of eight main natural polyphenols (caffeic, p-coumaric, dihydroxybenzoic and gallic acid, rutin, resveratrol, quercetin and catechin) was possible by combining MSPD and capillary liquid chromatography coupled to a diode array detection and a mass simple quadrupole analyzer (cLC-DAD-MS). Good linearity and acceptable LOD (0.05–62 µg·g−1) and LOQ (0.2–207 µg·g−1) were obtained. The quantities of extracted polyphenols were within 2.4 and 333 µg·g−1, with catechin and rutin the most abundant compounds in grape pomace and grape wastes, respectively. Furthermore, considering the prospective uses of the winery bioresidues, the extracts have been characterised in terms of bioactive properties (several antioxidant activities and bacterial inhibition against Staphylococcus aureus, Escherichia coli and Pseudomona aeruginosa) and parameters such as total polyphenol and total flavonoid content. The high antioxidant activity (IC50 5.0 ± 0.4 µg ·g−1 against DPPH radical) and antibacterial activity (2.2 ± 0.3 mg·mL−1) suggests that the methodology developed is efficient, rapid and promising for the extraction of phenolic compounds with potential application as bioactive ingredients in food and cosmetic industries.
ISSN:0021-9673
DOI:10.1016/j.chroma.2021.462128