Role of configurational flexibility on the adsorption kinetics of bivalent bispecific antibodies on porous cation exchange resins

• Published in: Journal of Chromatography A 2021-10, Vol.1655, p.462479, Article 462479
• Main Authors: Kimerer, Lucas K., Pabst, Timothy M., Hunter, Alan K., Carta, Giorgio
• Format: Article
• Language: English
• Subjects: Bispecific bivalent antibodies; Confocal microscopy; Conformational interconversion; Ion exchange; Mass transfer modeling
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2021.462479

•Kinetics of adsorption of bi-valent bi-specific antibodies studied via CLSM.•Kinetically-limited interconversion of bound forms impacts adsorption kinetics.•pH, ionic strength, and temperature influence intraparticle bound protein profiles.•Model describes interplay of diffusion, adsorption and conformational interconversion. The adsorption kinetics of a monoclonal antibody (mAb) used as a reference and of bivalent bispecific antibodies (BiSAb) on a macroporous cation exchanger is studied experimentally by examining the transient patterns of bound protein within the particles using confocal microscopy for a range of protein concentrations, buffer concentrations and pH, and temperatures. The mAb adsorption kinetics is controlled by pore diffusion and conforms to the classical shrinking core model. While the mAb adsorption rate increases with temperature, the ratio of effective and free solution diffusivity, De /D0, remains constant and has a value of 0.20. The BiSAb's structure is comprised of scFv domains that are genetically fused to a framework IgG through flexible peptide linkers which results in conformational flexibility leading to multiple binding forms with varying affinity for the adsorbent surface. As a result, adsorption of the BiSAbs shows complex patterns of total bound protein within the particles. These BiSAb adsorption patterns are influenced by buffer ionic strength, pH, and temperature in unique ways. Sharper intraparticle profiles are observed for conditions where the binding strength is greater (lower buffer concentration and/or pH) or when the protein is chemically crosslinked to restrict configurational flexibility. Temperature affects the BiSAb pore diffusivity as well as the interconversion kinetics. While the effects of temperature on BiSAb transport are also described by a constant De /D0 = 0.15, the temperature also affects the rate of interconversion between binding forms leading to faster equilibration at higher temperatures. A phenomenological model indicates that the interplay of pore diffusion and adsorption with the kinetically limited interconversion between binding forms is responsible for the experimental trends.