Separation of inorganic anions on reversed-phase C18 columns with a phosphomolybdate mobile phase

• Published in: Journal of Chromatography A 2024-05, Vol.1722, p.464843, Article 464843
• Main Authors: Kemmei, Tomoko, Yudo, Azusa, Kodama, Shuji, Yamamoto, Atsushi, Inoue, Yoshinori, Kagaya, Shigehiro, Hayakawa, Kazuichi
• Format: Article
• Language: English
• Subjects: Inorganic anions; Phosphomolybdate; Reversed-phase liquid chromatography; Ultraviolet detection
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2024.464843

•A simple RP-HPLC/UV method for the analysis of inorganic anions was developed.•Inorganic anions were separated using a reversed-phase C18 column.•Adding molybdate to phosphoric acid mobile phase made the UV detection of inorganic anions possible.•The method successfully determined eight inorganic anions.•The method accurately measured inorganic anions in some water samples. Reversed-phase high performance liquid chromatography (RP-HPLC) is the most widely used chromatographic method. In addition to hydrophobic interactions, additional interactions such as electrostatic interactions may participate in the retention behaviour of an analyte. This makes it possible to use RP-HPLC for many types of analyte. We describe a simple method for separating inorganic anions on a C18 column, in which retention of inorganic anions is almost entirely due to electrostatic interactions. This leads to rapid separations as well as higher theoretical plate numbers. We used 2 mM phosphoric acid containing a low concentration of disodium molybdate as the mobile phase, which allows UV detection of non-UV-absorbing anions. With this method, we determined eight inorganic anions including several non-UV-absorbing anions photometrically at 220 nm. The detection limits of the examined eight inorganic anions calculated at a signal-to-noise ratio of 3 were between 0.3 and 10 μM. The detector response was linear over three orders of magnitude of inorganic anion concentration. The proposed RP-HPLC/UV method was successfully applied to determine inorganic anions in some water samples.