Simultaneous qualitative and quantitative analysis to explore the material basis for different formulations of Dachengqi decoction to produce different efficacy by UPLC-QTOF-MS and UFLC-QQQ-MS

• Published in: Fēnxī huàxué 2024-12, p.100488, Article 100488
• Main Authors: Li, Hai-Zhen, Lou, Yan, Shu, Ying-Ying, Jin, Wan-Ting, Yao, Xiao-Xuan, Song, Jie, Chen, Yin-Fang, Nie, Bin
• Format: Article
• Language: English
• Subjects: Component content; Dachengqi Decoction; Material basis; Quality control; Quantitative detection; UFLC-QQQ-MS; UPLC-Q-TOF-MS
• ISSN: 1872-2040
• DOI: 10.1016/j.cjac.2024.100488

•A comprehensive and rapid method for quality evaluation and control of Dachengqi Decoction (DCQD) was developed by integrating UPLC-QTOF-MS and UFLC-QQQ-MS.•A total of 190 constituents were tentatively identified in qualitative analysis, of which 27 compounds were unambiguously confirmed by comparing with reference substances.•19 important constituents were determined simultaneously within 10 min by multiple reaction monitoring (MRM).•The validated method was applied for determining ten different proportion DCQDs. It was found that different proportions generated great influence on dissolution of constituents, which was believed to be one of the main mechanisms of the synergistic effect of the medicinal ingredients in DCQD. Dachengqi Decoction (DCQD) is a well-known prescription of catharsis in “Shang Han Lun”, composed of four traditional Chinese medical ingredients: Radix et Rhizoma Rhei (Dahuang), Cortex Magnoliae officinalis (Houpo), Fructus Aurantii Immaturus (Zhishi) and Natrii Sulfas (Mangxiao). Due to the complexity of its composition and inconsistencies in the traditional decocting process, maintaining the quality and exploring the material basis for efficacy of DCQD is challenging. In this study, we established an integrating ultra-high-performance liquid chromatography equipped with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) and ultra-fast-performance liquid chromatography equipped with triple quadrupole mass spectrometry (UFLC-QQQ-MS) method to perform qualitative and quantitative analyses of different DCQD formulations. The methods of quality control and content detection of the main components of different formulations were improved by optimizing the parameters of mobile phase composition, gradient and velocity. By optimizing of the method, the separation ability of structurally similar substances such as aloe-emodin, emodin and Apigenin is greatly improved. As a result, in the qualitative analysis, 190 components were detected of which 27 compounds were unambiguously identified by comparison with reference compounds by chromatographic behavior and mass spectrum, and the remaining compounds were tentatively assigned by comparison with fragmentation pathways and characteristic fragment ions in published literature or known databases. In the quantitative analysis, the contents of 19 key ingredients across 10 formulations were determined. The results showed that some components were roughly distributed according to the proportio