Cytocompatibility and uptake of polycations-modified halloysite clay nanotubes

The increasing use of clay-based polymer composite materials in industry and biomedicine stimulates the investigation of cellular uptake and toxicity of polymer\clay composites. In particular, halloysite nanotubes have become a promising material for a number of applications. Among other polymers, c...

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Bibliographic Details
Published in:Applied clay science 2019-03, Vol.169, p.21-30
Main Authors: Tarasova, Evgenya, Naumenko, Ekaterina, Rozhina, Elvira, Akhatova, Farida, Fakhrullin, Rawil
Format: Article
Language:English
Subjects:
Cellular uptake
Cytotoxicity
Halloysite nanotubes (Hal)
Layer-by-Layer assembly
Polyelectrolytes
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Summary:The increasing use of clay-based polymer composite materials in industry and biomedicine stimulates the investigation of cellular uptake and toxicity of polymer\clay composites. In particular, halloysite nanotubes have become a promising material for a number of applications. Among other polymers, cationic polyelectrolytes have been employed in numerous studies as halloysite surface modifiers, without paying the proper attention to their toxicity. In this study we report our results on cytocompatibility and uptake of polycations-coated halloysite nanotubes. Adsorption of polycations onto clay nanotubes was investigated using microscopy and dynamic light scattering. We investigated cellular distribution and biological effects of halloysite surface-modified with poly(ethylenimine), poly(diallyldimethyl-ammonium) and poly (allylamine). We have employed a set of biological methods, such as microscopy (dark-field, electron and atomic force) and enzymatic tests to assay the toxicity of polycations-coated halloysite, using A549 human endothelial carcinoma cells in 2D and 3D cultures as the biological model. We found that polycations adsorbed onto halloysite surfaces significantly increase the otherwise low toxicity of pristine clay nanotubes, inducing cellular and nuclear defects. •Halloysite clay nanotubes were coated with a single layer of biomedically-relevant polycations•A549 humal lung carcinoma cells in culture and as multicellular spheroids were used to assay the toxicity of cations-coated halloysite•A set of microscopic and enzymatic tests was employed to assay the cytotoxicity and uptake of polycations-coated clay nanotubes
ISSN:0169-1317
1872-9053
DOI:10.1016/j.clay.2018.12.016