Formulation of O-carboxymethyl chitosan with magnesium phosphate cement promotes in vitro/in vivo angiogenesis and osteogenesis related to the TRPM7 channel in bone regeneration

• Published in: Composites. Part B, Engineering Engineering, 2024-07, Vol.280, p.111451, Article 111451
• Main Authors: Gong, Changtian, Yang, Jian, Zheng, Di, Zhou, Bin, Zhang, Xiping, Wang, Xingyu, Huang, Xinghan, Ye, Qingsong, Guo, Weichun
• Format: Article
• Language: English
• Subjects: Bone regeneration; Magnesium phosphate cement; O-Carboxymethyl chitosan; Periodontitis; TRPM7 channel
• ISSN: 1359-8368; 1879-1069
• DOI: 10.1016/j.compositesb.2024.111451

Despite the abundant evidence on the biodegradability of K-struvite (KMgPO4·6H2O) that promotes bone regeneration, the cell-related surface bioactivity still stays low owing to the absence of organic components. O-Carboxymethyl chitosan (O-CMC) is a glycosaminoglycan-mimetic with outstanding biocompatibility, biodegradability and solubility, which has been successfully used in tissue engineering and regenerative medicine (TERM). In this study, we incorporated O-CMC into K-struvite to prepare hybrid scaffolds (OMPCs) with increasing biphasic contents. We found that O-CMC improved the physicochemical properties, in vitro/vivo biocompatibility, and enzymatic biodegradability of K-struvite, resulting in enhanced in vitro/vivo angiogenesis and osteogenesis via the proper release of Mg2+. In addition, we found differential expression of transient receptor potential cation channel member 7 (TRPM7) within the endothelial and osteoblast lineages, triggering the phosphorylation of the PI3K/Akt and MEK/ERK signaling pathways, respectively. In summary, it can be concluded that the biphasic addition of O-CMC is a viable method for improving the surface bioactivity of K-struvite to accelerate bone regeneration. •Novel hybrid scaffolds (OMPCs) with improved biodegradability and surface bioactivity were successfully developed.•OMPCs systematically enhance in vitro/in vivo angiogenesis and osteogenesis in bone regeneration.•Regulatory mechanisms of OMPCs in angiogenesis and osteogenesis are related to TRPM7 channels.