Linking glucose oxidation to luminol-based electrochemiluminescence using bipolar electrochemistry

• Published in: Electrochemistry communications 2015-01, Vol.50, p.77-80
• Main Authors: Eßmann, Vera, Jambrec, Daliborka, Kuhn, Alexander, Schuhmann, Wolfgang
• Format: Article
• Language: English
• Subjects: Bipolar electrochemistry; Electrochemiluminescence; Glucose oxidase; Luminol; Prussian blue
• ISSN: 1388-2481; 1873-1902
• DOI: 10.1016/j.elecom.2014.11.015

The range of potential analytes for bipolar electrochemistry can be significantly extended by modification of bipolar electrodes with enzymatic biosensing layers. In this study, we employed a Prussian blue-based glucose detection system involving electrochemical reduction of enzymatically generated hydrogen peroxide at the cathodic pole. The concentration of glucose in solution can be correlated with oxidative luminol electrochemiluminescence at the opposite anodic pole, which was recorded with a photomultiplier tube. This opens a route for novel analytical systems using glucose oxidase as an amplification element for the reporter reaction. [Display omitted] •Combination of an immobilised enzymatic biosensor and bipolar electrochemistry•Reductive glucose detection using glucose oxidase and a layer of Prussian blue on the cathodic pole of the bipolar electrode•Oxidative luminol electrochemiluminescence used to infer the amount of analyte at the cathodic pole•Development of a universal setup for the general investigation of novel experimental concepts for bipolar electrochemistry