Genetic associations uncover candidate SNP markers and genes associated with salt tolerance during seedling developmental phase in barley

• Published in: Environmental and experimental botany 2021-08, Vol.188, p.104499, Article 104499
• Main Authors: Thabet, Samar G., Moursi, Yasser S., Sallam, Ahmed, Karam, Mohamed A., Alqudah, Ahmad M.
• Format: Article
• Language: English
• Subjects: Barley; Candidate genes; GWAS; Potassium channel; Salinity
• ISSN: 0098-8472; 1873-7307
• DOI: 10.1016/j.envexpbot.2021.104499

•Genetic basis of natural variation of germination and seedling-related traits under salt stress in worldwide barley collection.•Uncover the genetic factors influencing germination and seedling performance-related traits using 9K SNPs.•Novel stage- and trait-specific associations were detected by GWAS analysis.•Salt stress-responsive candidate genes encoding different proteins that regulate the studied traits were identified.•Interestingly, many candidate genes are potentially involved in salt tolerance e.g. potassium channel and Squamosa promoter-binding-like protein 6. Understanding the genetic basis of seed germination under salt stress as severe abiotic stress can help in improving barley plant growth performance, production, and productivity. Salt tolerance is complex trait that controlled by many genes and its genetic basis needs to be dissected. In the current study, 121 spring worldwide barley accessions were evaluated for salinity stress tolerance using 200 mM NaCl during seed germination and seedling developmental phase. Induced salt stress reduced the seed germination parameters and seedling related-traits significantly. The genome-wide association scan (GWAS) was applied using 9K SNPs and revealed several interesting genomic regions including 80 significant SNPs associated with the studied traits. The genomic regions of these SNPs harbored around 1500 candidate genes within the linkage disequilibrium interval. Interestingly, many genes encoding potassium channel were mapped e.g. AKT2 on chromosomes 1H. Genes identified in the current study were found to be linked to salinity stress tolerance during seedling developmental phase as a first time such as Squamosa promoter-binding-like protein 6 at chromosome 5H. Here, we demonstrated their putative role in salt tolerance at early developmental stages indicating that selection at early stages would be reliable to improve salinity tolerance. Functional markers linked with the resistance loci would be useful for breeding to improve barley cultivars with enhanced tolerance to salt stress. Further genetic investigation on these loci and their linked genes would provide insight into understanding the molecular mechanisms of salt stress in barley.