Mitochondrial proteomics profile points oxidative phosphorylation as main target for beauvericin and enniatin B mixture

• Published in: Food and chemical toxicology 2020-07, Vol.141, p.111432, Article 111432
• Main Authors: Alonso-Garrido, M., Manyes, L., Pralea, I.E., Iuga, C.A.
• Format: Article
• Language: English
• Subjects: Depsipeptides - administration & dosage; Depsipeptides - pharmacology; Electron Transport; Electron transport chain; Humans; In vitro; Jurkat Cells; Lymphocyte; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondrial Proteins - metabolism; Mixture; Mycotoxin; Oxidative Phosphorylation; Proteomics; Transcription, Genetic - drug effects
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2020.111432

Beauvericin (BEA) and enniatin B (EN B) are non-legislated Fusarium mycotoxins usually found in cereal and cereal-based products all around the world. By the proteomic analysis of mitochondria enriched extracts from Jurkat cells exposed for 24 h to three concentrations of BEA:EN B (0.01–0.1–0.5 μM), a number of 1821 proteins (202 mitochondrial) were identified and relatively quantified. 340 proteins (59 mitochondrial) were statistically significant altered in our samples (Anova p-value ≤ 0.05 and fold change (FC) ≥1.5). The protein mitochondrial translational release factor 1 like (MTRF1L) was the most abundant protein in the three mycotoxin exposures studied. The mycotoxins mixture exposure induced concentration dependent changes at mitochondrial proteins levels that mainly involve inner and outer membrane complexes, Electron Transport Chain (ETC) and ribosomes. These results showed alteration of proteins levels related to oxidative phosphorylation, metabolic and neurodegenerative diseases related pathways. •EN B and BEA mixture provoques mitochondrial toxicity at proteomic level.•Proteomics analysis revealed 340 proteins significative altered, 59 mitochondrial.•Oxidative phosporylation pathway analysis reported 24 altered genes.