Endocannabinoid, anandamide in gingival tissue regulates the periodontal inflammation through NF-κB pathway inhibition

Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly...

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Bibliographic Details
Published in:FEBS letters 2006-01, Vol.580 (2), p.613-619
Main Authors: Nakajima, Yumiko, Furuichi, Yasushi, Biswas, Kamal Krishna, Hashiguchi, Teruto, Kawahara, Ko-ichi, Yamaji, Kazuyo, Uchimura, Tomonori, Izumi, Yuichi, Maruyama, Ikuro
Format: Article
Language:English
Subjects:
AEA
Anandamide
Cannabinoid receptor
Cytokine
electrophoretic mobility shift assay
EMSA
GAPDH
GCF
gingival crevicular fluid
glyceraldehyde-3-phosphate dehydrogenase
HGFs
high-performance liquid chromatography
HPLC
human gingival fibroblasts
N-arachidonoyl ethanolamine
NF-κB
Periodontitis
PMB
polymyxin B
Porphyromonas gingivalis
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Summary:Anandamide (AEA) exhibits anti-inflammatory effects. However, its role in the periodontal field remains unknown. Here, we found that gingival crevicular fluid contained a detectable level of AEA. The cannabinoid receptors CB1 and CB2 were expressed by human gingival fibroblasts (HGFs), and markedly upregulated under pathological conditions. AEA significantly reduced the production of pro-inflammatory mediators (IL-6, IL-8 and MCP-1) induced by Porphyromonas gingivalis LPS in HGFs, and this effect was attenuated by AM251 and SR144528, selective antagonists of CB1 and CB2, respectively. Moreover, AEA completely blocked LPS-triggered NF-κB activation, implying that AEA may regulate hyperinflammatory reactions in periodontitis.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2005.12.079