Inhibitory potential of trilobatin from Lithocarpus polystachyus Rehd against α-glucosidase and α-amylase linked to type 2 diabetes

► We identified the sweet compound from Lithocarpus polystachyus Rehd as trilobatin. ► We evaluated inhibitory activity of trilobatin against α-glucosidase and α-amylase. ► Trilobatin inhibits α-glucosidase strongly and α-amylase mildly. ► The DPPH scavenging activity was evaluated and trilobatin wa...

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Bibliographic Details
Published in:Food chemistry 2012, Vol.130 (2), p.261-266
Main Authors: Dong, Hua-Qiang, Li, Mei, Zhu, Feng, Liu, Fu-Lai, Huang, Jian-Bo
Format: Article
Language:English
Subjects:
acarbose
adverse effects
alpha-amylase
alpha-glucosidase
animals
antioxidant activity
chemical structure
clinical trials
high performance liquid chromatography
hyperglycemia
Lithocarpus
Lithocarpus polystachyus Rehd
noninsulin-dependent diabetes mellitus
nuclear magnetic resonance spectroscopy
rutin
Trilobatin
Type 2 diabetes
α-Amylase
α-Glucosidase
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Summary:► We identified the sweet compound from Lithocarpus polystachyus Rehd as trilobatin. ► We evaluated inhibitory activity of trilobatin against α-glucosidase and α-amylase. ► Trilobatin inhibits α-glucosidase strongly and α-amylase mildly. ► The DPPH scavenging activity was evaluated and trilobatin was higher than rutin. The chemical structure of the sweet compound from Lithocarpus polystachyus Rehd was identified as trilobatin on the basis of HPLC, EIS-MS and NMR analyses. The inhibitory activities of trilobatin against α-glucosidase and α-amylase were evaluated, and the inhibition mechanism was analysed with Lineweaver–Burk plots. Also the antioxidant activity evaluation of trilobatin was conducted by DPPH radical scavenging assay. Comparing with acarbose, trilobatin showed a strong inhibitory activity against α-glucosidase and a moderate inhibitory activity against α-amylase. The Lineweaver–Burk plots analysis elucidated that trilobatin inhibited the enzyme non-competitively. DPPH scavenging activity of trilobatin (IC 50 = 0.57 mg/ml) was higher than rutin (IC 50 = 0.72 mg/ml), which indicated that trilobatin had a moderate antioxidant potential. These results suggest that trilobatin is a potential effective α-glucosidase inhibitor for management of postprandial hyperglycemia with less side effect, and provide strong rationale for further animal and clinical studies.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2011.07.030