Protein-lipid composite nanoparticles for the oral delivery of vitamin B12: Impact of protein succinylation on nanoparticle physicochemical and biological properties

• Published in: Food hydrocolloids 2019-07, Vol.92, p.189-197
• Main Authors: Liu, Guangyu, Yang, Jingqi, Wang, Yixiang, Liu, Xinghai, Guan, Le Luo, Chen, Lingyun
• Format: Article
• Language: English
• Subjects: Biological response; Oral delivery; Protein-lipid composite nanoparticle; Succinylation; Surface properties; Vitamin B12
• ISSN: 0268-005X; 1873-7137
• DOI: 10.1016/j.foodhyd.2018.12.020

Protein-lipid composite nanoparticles featuring three-layer structure (protein layer, α-tocopherol layer and phospholipid layer) and an inner aqueous compartment have been developed recently as a delivery system for hydrophilic nutraceuticals. These composite nanoparticles overcome many shortfalls of sole protein or lipid nanoparticles, however, exhibited poor stability during storage and high burst release in the intestinal environment. In this study, the performance of these nanoparticles was improved by modifying the protein outer layer through succinylation. The increased surface charge and spatial extension of succinate chain on nanoparticle surface improved the nanoparticle stability in both physiological buffer and water. The crosslinking by succinate minimized the leakage of vitamin B12 to 4.5% during one month of storage. Moreover, succinylation slowed down pancreatic digestion of the protein shell, leading to a sustainable release within 10 h in the simulated intestinal fluid. Also, the modified nanoparticle increased the uptake efficiency of vitamin B12 over 20 folds and demonstrated a good mucoadhesive capacity. The in vivo efficacy study showed that the modified nanoparticle could correct a vitamin B12 deficiency in a rat model more efficiently than a free vitamin B12 supplement. [Display omitted] •Succinylation increased the stability of nanoparticles.•Succinylation improved the release behavior of nanoparticles in the intestinal environment.•Nanoparticles with succinylation modification demonstrated high cellular uptake efficiency and good mucoadhesive capacity.•Such nanoparticles improved the oral absorption of vitamin B12in vivo.