Lutein and zeaxanthin supplementation reduces photooxidative damage and modulates the expression of inflammation-related genes in retinal pigment epithelial cells

• Published in: Free radical biology & medicine 2012-09, Vol.53 (6), p.1298-1307
• Main Authors: Bian, Qingning, Gao, Shasha, Zhou, Jilin, Qin, Jian, Taylor, Allen, Johnson, Elizabeth J., Tang, Guangwen, Sparrow, Janet R., Gierhart, Dennis, Shang, Fu
• Format: Article
• Language: English
• Subjects: blood serum; blue light; Cells, Cultured; Chemokine CCL2 - genetics; Chemokine CCL2 - metabolism; Complement Factor H - genetics; Complement Factor H - metabolism; Culture Media; Dietary Supplements; Down-Regulation - drug effects; Down-Regulation - radiation effects; epidemiological studies; epithelial cells; Gene Expression - drug effects; Gene Expression - radiation effects; genes; Humans; Inflammation; Inflammation Mediators - metabolism; Interleukin-6 - genetics; Interleukin-6 - metabolism; interleukin-8; Interleukin-8 - genetics; Interleukin-8 - metabolism; Lutein; Lutein - metabolism; Lutein - pharmacology; macular degeneration; Macular Degeneration - drug therapy; Macular Degeneration - pathology; Oxidation-Reduction; Oxidative Stress - radiation effects; pathogenesis; Photochemical Processes; Photooxidation; Proteasome; proteasome endopeptidase complex; Proteasome Endopeptidase Complex - metabolism; protective effect; Radiation-Protective Agents - metabolism; Radiation-Protective Agents - pharmacology; Retinal Pigment Epithelium - drug effects; Retinal Pigment Epithelium - metabolism; Retinal Pigment Epithelium - pathology; risk; RPE; Ultraviolet Rays; Xanthophylls - metabolism; Xanthophylls - pharmacology; Zeaxanthin; Zeaxanthins
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2012.06.024

Oxidative damage and inflammation are related to the pathogenesis of age-related macular degeneration (AMD). Epidemiologic studies suggest that insufficient dietary lutein and zeaxanthin intake or lower serum zeaxanthin levels are associated with increased risk for AMD. The objective of this work is to test the protective effects of lutein and zeaxanthin against photooxidative damage to retinal pigment epithelial cells (RPE) and oxidation-induced changes in expression of inflammation-related genes. To mimic lipofuscin-mediated photooxidation in vivo, we used ARPE-19 cells that accumulated A2E, a lipofuscin fluorophore and photosensitizer, as a model system to investigate the effects of lutein and zeaxanthin supplementation. The data show that supplementation with lutein or zeaxanthin in the medium resulted in accumulation of lutein or zeaxanthin in the RPE cells. The concentrations of lutein and zeaxanthin in the cells were 2- to 14-fold of that detected in the medium, indicating that ARPE-19 cells actively take up lutein or zeaxanthin. As compared with untreated cells, exposure of A2E-containing RPE to blue light resulted in a 40–60% decrease in proteasome activity, a 50–80% decrease in expression of CFH and MCP-1, and an∼20-fold increase in expression of IL-8. The photooxidation-induced changes in expression of MCP-1, IL-8, and CFH were similar to those caused by chemical inhibition of the proteasome, suggesting that inactivation of the proteasome is involved in the photooxidation-induced alteration in expression of these inflammation-related genes. Incubation of the A2E-containing RPE with lutein or zeaxanthin prior to blue light exposure significantly attenuated the photooxidation-induced inactivation of the proteasome and photooxidation-induced changes in expression of MCP-1, IL-8, and CFH. Together, these data indicate that lutein or zeaxanthin modulates inflammatory responses in cultured RPE in response to photooxidation. Protecting the proteasome from oxidative inactivation appears to be one of the mechanisms by which lutein and zeaxanthin modulate the inflammatory response. Similar mechanisms may explain salutary effects of lutein and zeaxanthin in reducing the risk for AMD. ► Photooxidation inactivates the proteasome in retina pigment epithelial cells (RPE). ► Inactivation of the proteasome alters the expression of inflammation-related genes. ► RPE cells accumulate lutein and zeaxanthin on supplementation. ► Lutein or zeaxanthin attenuates photoo