Imaging mass spectrometry as a novel Cys-rich peptide detection technique in plant tissue

A successful novel method for detecting Cys-rich peptides in plant tissue using MALDI-IMS is first reported. The novel method provides a rapid and robust screening method for peptide detection because extraction and purification processes are not required. In addition, the novel method also provides...

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Bibliographic Details
Published in:International journal of mass spectrometry 2022-07, Vol.477, p.116857, Article 116857
Main Authors: Niyomploy, Ploypat, Sangvanich, Polkit
Format: Article
Language:English
Subjects:
Cyclotide
Imaging mass spectrometry
MALDI-TOF
Plant tissue
SEM
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Summary:A successful novel method for detecting Cys-rich peptides in plant tissue using MALDI-IMS is first reported. The novel method provides a rapid and robust screening method for peptide detection because extraction and purification processes are not required. In addition, the novel method also provides greater convenience for peptide characterization and determining peptide localization because it can perform these steps directly on tissue, resulting in the use of 10- to 20-fold less plant sample compared to that required for conventional methods. As a result, the novel method is successful for peptide detection in tissue and is probably applicable for the detection of other active Cys-rich peptides. [Display omitted] •We are successful to develop the novel method for detecting Cys-rich peptides in plant tissue using MALDI-IMS.•The novel method provides greater convenience for peptide characterization and determining peptide localization.
ISSN:1387-3806
1873-2798
DOI:10.1016/j.ijms.2022.116857