Solid and liquid fractionation of sugarcane and Agave bagasse during ozonolysis and enzymatic hydrolysis: Impact on biohydrogen and biogas production

• Published in: Industrial crops and products 2024-04, Vol.210, p.118175, Article 118175
• Main Authors: Pérez-Barragán, Jacobo, García-Depraect, Octavio, Maya-Yescas, Rafael, Vallejo-Rodríguez, Ramiro, Palacios-Hinestroza, Hasbleidy, Coca, Mónica, Castro-Muñoz, Roberto, León-Becerril, Elizabeth
• Format: Article
• Language: English
• Subjects: Agave tequilana; Biofuels; Dark fermentation; Lignocellulosic biomass; Ozonolysis
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2024.118175

Sustainable and cost-efficient lignocellulosic biomass-to-energy bioprocesses require assessing the main components released during biomass fractionation. This study assessed the mesophilic biochemical potential of biohydrogen (BHP) and methane production (BMP) from sugarcane bagasse (SCB) and cooked (CAB) and uncooked (UAB) Agave bagasse. A systematic comparative analysis was performed for the liquid and solid fractions derived from classical enzymatic hydrolysis and ozonolysis-assisted enzymatic hydrolysis. Ozonolysis partially delignified the materials tested while preserving most fermentable sugars and avoiding the generation of inhibitors. During hydrolysis, ozonolysis also led to higher sugar yields and organic matter solubilization regardless of the bagasse type. Hydrolysates with ozonated material showed the highest BHP of 135, 94 and 105 NmL-H2/g-VSfed and BMP of 250, 269 and 247 NmL-CH4/g-VSfed, for SCB, CAB, and UAB, respectively. The total amount of energy harvested was dependent on the material tested and the biomass conversion process applied. Compared with raw material, ozonolysis, followed by enzymatic hydrolysis, increased both the BHP and BMP by 153–371% and 5–53%, respectively. [Display omitted] •Ozonolysis changed the structural composition of lignocellulosic bagasse.•O3 led to higher COD solubilization and sugar recovery during saccharification.•Ozonated hydrolysates led to up to 78.2% more H2 yield compared to raw bagasse.•Average CH4 yield of 260 NmL CH4/g VSfed with ozonated enzymatic hydrolysates.•Besides hydrolysates, the residual bagasse fibers had good potential for CH4.