Derivatization/chromophore introduction of tranexamic acid and its HPLC determination in pharmaceutical formulations

• Published in: Journal of the Association of Arab Universities for Basic & Applied Sciences 2015-04, Vol.17 (1), p.51-56
• Main Authors: Ashfaq, M., Aslam, A., Mustafa, G., Danish, M., Nazar, M. Faizan, Asghar, M. Nadeem
• Format: Article
• Language: English
• Subjects: Chromophore; Derivatives; Derivatization; High performance liquid chromatography; HPLC; Tranexamic acid; Validation
• ISSN: 1815-3852; 1815-3852
• DOI: 10.1016/j.jaubas.2014.02.005

A viable cost-effective and isocratic approach employing C-18 column (250mm×4.6mm, 5μm) based HPLC has been utilized to separate and estimate the drug, tranexamic acid in pharmaceutical formulations. Tranexamic acid contains no π-electrons to act as fluorophore or chromophore hence pre-column derivatization was performed with benzene sulfonyl chloride in aqueous medium at room temperature. The derivatized drug was then estimated using C-18 column by exploiting a 25:75 (v/v) solvent mixture of acetonitrile and 0.1M ammonium acetate (pH 5.0) as the mobile phase. The flow rate of mobile phase was 1mL/min and detection was performed at a wavelength of 232nm using UV detector. Retention time of tranexamic acid was 4.42min. The method followed linear regression equation in the concentration range of 1–100μg/mL with co-efficient of determination equal to 0.9994. The limit of detection and limit of quantitation were 0.3 and 1μg/mL, respectively. The relative standard deviation and recovery ranges for tranexamic acid were found to be 0.11–2.47% and 97.60–103.25%, respectively. The suggested method is very sensitive and may have the potential to be used for tranexamic acid detection in medicinal formulations.