Inactivation kinetics of dairy phages using photocatalytic paint under diverse environmental conditions and radiation sources

• Published in: Journal of environmental chemical engineering 2024-12, Vol.12 (6), p.114261, Article 114261
• Main Authors: Jacob, María Fiorella, Alfano, Orlando Mario, Quiberoni, Andrea del Luján, Briggiler Marcó, Mariángeles, Ballari, María de los Milagros
• Format: Article
• Language: English
• Subjects: Kinetic model; Lactic acid bacteria; Phage inactivation; Photocatalysis; Radiation flux; Relative humidity
• ISSN: 2213-3437
• DOI: 10.1016/j.jece.2024.114261

This article investigates the efficacy of a photocatalytic paint for bacteriophages inactivation under various levels of incident radiation flux, relative humidity and radiation sources. Two phages that infect lactic acid bacteria were selected: J-1, infective of Lacticaseibacillus casei ATCC 27139, and M13-G1b, infective of Streptococcus thermophilus G1b. Phage suspensions were deposited on coated and uncoated plates and exposed to radiation typically used for indoor illumination (λ = 360–720 nm). A first-order inactivation kinetics was proposed with respect to the Plaque Forming Units per mL (PFU/mL) that correlates the inactivation constant with the operating conditions. The kinetic constant of this novel model combines a potential function for the incident radiation flux and a logistic function for the relative humidity. The results showed that as these variables increased, phage inactivation turned higher for both uncoated and coated plates. Photonic and quantum efficiencies were also calculated for both phages, when treated with photocatalytic paint under UV-A radiation. The photonic efficiencies were 8.03 × 1012 PFU/Einstein for J-1 and 4.05 × 1012 PFU/Einstein for M13-G1b, while the quantum efficiencies were 1.19 × 1013 PFU/Einstein for J-1 and 6.03 × 1012 PFU/Einstein for M13-G1b. Good agreement was obtained between predictions of the proposed kinetic model and experimental data, with a Root Mean Square Error of 4.43 % for J-1 and 2.98 % for M13-G1b. The developed model would be useful to predict phage inactivation under diverse combinations of operating conditions levels in environments of dairy plants, different from those tested in this work. [Display omitted] •Dairy phage inactivation using photocatalytic paint was studied.•Influence of relative humidity, radiation flux and radiation source was assayed.•Phage inactivation increased with both radiation flux and relative humidity.•Inactivation efficiencies in presence of UV-A radiation were higher.•Experimental data well-fitted by kinetic model predictions.