Inhibition of bacterial oxidation of ferrous iron by lead nitrate in sulfate-rich systems

• Published in: Journal of hazardous materials 2013-01, Vol.244-245, p.718-725
• Main Authors: Wang, Hongmei, Gong, Linfeng, Cravotta, Charles A., Yang, Xiaofen, Tuovinen, Olli H., Dong, Hailiang, Fu, Xiang
• Format: Article
• Language: English
• Subjects: A. ferrooxidans; Acid mine drainage; Acidithiobacillus - drug effects; Acidithiobacillus - metabolism; Anglesite; Ferrous Compounds - metabolism; Hydrogen-Ion Concentration; Industrial Waste; Iron - metabolism; Iron oxidation; Jarosite; Lead - toxicity; Mining; Nitrates - toxicity; Oxidation-Reduction
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2012.11.004

► Acidithiobacillus ferrooxidans was grown with Fe2+ in the presence of 0–24mM Pb(NO3)2. ► Jarosite precipitated upon iron oxidation but Pb-jarosite was not detected. ► Pb precipitated as anglesite and dissolved Pb remained below 20μM levels. ► Bacterial iron oxidation was inhibited by nitrate (up to 48mM) instead of Pb. ► The lack of Pb-jarosite was consistent with the PHREEQC geochemical model. Inhibition of bacterial oxidation of ferrous iron (Fe(II)) by Pb(NO3)2 was investigated with a mixed culture of Acidithiobacillus ferrooxidans. The culture was incubated at 30°C in ferrous-sulfate medium amended with 0–24.2mM Pb(II) added as Pb(NO3)2. Anglesite (PbSO4) precipitated immediately upon Pb addition and was the only solid phase detected in the abiotic controls. Both anglesite and jarosite (KFe3(SO4)2(OH)6) were detected in inoculated cultures. Precipitation of anglesite maintained dissolved Pb concentrations at 16.9–17.6μM regardless of the concentrations of Pb(NO3)2 added. Fe(II) oxidation was suppressed by 24.2mM Pb(NO3)2 addition even when anglesite was removed before inoculation. Experiments with 0–48mM KNO3 demonstrated that bacterial Fe(II) oxidation decreased as nitrate concentration increased. Therefore, inhibition of Fe(II) oxidation at 24.2mM Pb(NO3)2 addition resulted from nitrate toxicity instead of Pb addition. Geochemical modeling that considered the initial precipitation of anglesite to equilibrium followed by progressive oxidation of Fe(II) and the precipitation of jarosite and an amorphous iron hydroxide phase, without allowing plumbojarosite to precipitate were consistent with the experimental time-series data on Fe(II) oxidation under biotic conditions. Anglesite precipitation in mine tailings and other sulfate-rich systems maintains dissolved Pb concentrations below the toxicity threshold of A. ferrooxidans.